TY - JOUR
T1 - cDNA and genomic sequences for rat 8-oxo-dGTPase that prevents occurrence of spontaneous mutations due to oxidation of guanine nucleotides
AU - Cai, Jian Ping
AU - Kakuma, Tetsuya
AU - Tsuzuki, Teruhisa
AU - Sekiguchi, Mutsuo
N1 - Funding Information:
We thank Drs M.Furuichi and Y.Nakabeppu for discussion, and M.Ohara for helpful comments. This work was supported by grants from the Ministry of Education, Science and Culture, Japan, and HFSP.
PY - 1995/10
Y1 - 1995/10
N2 - The enzyme, 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase), is present In various organisms and plays an important role in control of spontaneous mutagenesis. This enzyme degrades 8-oxoguamne-contaning deoxyribonucleoside triphosphate, a potentially mutagenic substrate for DNA synthesis, to the corresponding monophosphate. To obtain appropriate probes for expression of the gene in various tissues and also to construct appropriate experimental models for carcino genesis, we cloned cDNA for rat 8-oxo-dGTPase and elucidated its structure. The nucleotide sequence of the cDNA revealed that the rat 8-oxo-dGTPase protein is composed of 156 amino acid residues. The molecular weight of rat 8-oxo-dGTPase, calculated from the predicted amino acid se was 18 006, and the 8-oxo-dGTPase protein of this size was detected when the cDNA was expressed in 8-oxo-dGTPase-deficlent Escherichitz coil mutT- cells. The predicted amino acid sequence of the rat 8-oxo-dGTPase has a close homology with those of human and bacterial counterparts. Using the cDNA as a probe, part of the rat gene for 8-oxo-dGTPase was isolated and was found to consist of at least three exons and spanned about 10 KB. A genomic region containing the pseudogene was also isolated.
AB - The enzyme, 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase), is present In various organisms and plays an important role in control of spontaneous mutagenesis. This enzyme degrades 8-oxoguamne-contaning deoxyribonucleoside triphosphate, a potentially mutagenic substrate for DNA synthesis, to the corresponding monophosphate. To obtain appropriate probes for expression of the gene in various tissues and also to construct appropriate experimental models for carcino genesis, we cloned cDNA for rat 8-oxo-dGTPase and elucidated its structure. The nucleotide sequence of the cDNA revealed that the rat 8-oxo-dGTPase protein is composed of 156 amino acid residues. The molecular weight of rat 8-oxo-dGTPase, calculated from the predicted amino acid se was 18 006, and the 8-oxo-dGTPase protein of this size was detected when the cDNA was expressed in 8-oxo-dGTPase-deficlent Escherichitz coil mutT- cells. The predicted amino acid sequence of the rat 8-oxo-dGTPase has a close homology with those of human and bacterial counterparts. Using the cDNA as a probe, part of the rat gene for 8-oxo-dGTPase was isolated and was found to consist of at least three exons and spanned about 10 KB. A genomic region containing the pseudogene was also isolated.
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U2 - 10.1093/carcin/16.10.2343
DO - 10.1093/carcin/16.10.2343
M3 - Article
C2 - 7586133
AN - SCOPUS:0028826044
SN - 0143-3334
VL - 16
SP - 2343
EP - 2350
JO - Carcinogenesis
JF - Carcinogenesis
IS - 10
ER -
