cDNA and genomic sequences for rat 8-oxo-dGTPase that prevents occurrence of spontaneous mutations due to oxidation of guanine nucleotides

Jian Ping Cai, Tetsuya Kakuma, Teruhisa Tsuzuki, Mutsuo Sekiguchi

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The enzyme, 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase), is present In various organisms and plays an important role in control of spontaneous mutagenesis. This enzyme degrades 8-oxoguamne-contaning deoxyribonucleoside triphosphate, a potentially mutagenic substrate for DNA synthesis, to the corresponding monophosphate. To obtain appropriate probes for expression of the gene in various tissues and also to construct appropriate experimental models for carcino genesis, we cloned cDNA for rat 8-oxo-dGTPase and elucidated its structure. The nucleotide sequence of the cDNA revealed that the rat 8-oxo-dGTPase protein is composed of 156 amino acid residues. The molecular weight of rat 8-oxo-dGTPase, calculated from the predicted amino acid se was 18 006, and the 8-oxo-dGTPase protein of this size was detected when the cDNA was expressed in 8-oxo-dGTPase-deficlent Escherichitz coil mutT- cells. The predicted amino acid sequence of the rat 8-oxo-dGTPase has a close homology with those of human and bacterial counterparts. Using the cDNA as a probe, part of the rat gene for 8-oxo-dGTPase was isolated and was found to consist of at least three exons and spanned about 10 KB. A genomic region containing the pseudogene was also isolated.

Original languageEnglish
Pages (from-to)2343-2350
Number of pages8
JournalCarcinogenesis
Volume16
Issue number10
DOIs
Publication statusPublished - Oct 1 1995

All Science Journal Classification (ASJC) codes

  • Cancer Research

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