cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco

Tatsurou Hamada, Takumi Nishiuchi, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba

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Abstract

A cDNA encoding the plastid ω-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desaturase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and increases the conversion of linoleic acid to linolenic acid in leaf polar lipids.

Original languageEnglish
Pages (from-to)606-611
Number of pages6
JournalPlant and Cell Physiology
Volume37
Issue number5
DOIs
Publication statusPublished - Jul 1996

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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