cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptides comparison among the insect circadian neuropeptides

Ayami Matsushima, Seiji Sato, Yoshiro Chuman, Yukimasa Takeda, Satoru Yokotani, Takeru Nose, Yoshiya Tominaga, Miki Shimohigashi, Yasuyuki Shimohigashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that of the cricket Gryllus bimaculatus and that of the last-summer cicada Meimuna opalifera. The G. bimaculatus PDF precursor is extraordinarily short and comprises a nuclear localization signal (NLS), while the M. opalifera PDF precursor is of ordinary length, comparable to that seen for the precursors of crustacean β-PDH homologues. Although their PDF peptide regions were exactly the same, the regions containing a signal peptide combined with a PDF-associated peptide (PAP) were remarkably different from each other. Such a grouping suggested a fundamental role for the PAP peptide in the circadian clock, perhaps associated with PDF function. In the present study, the cDNA cloning of PDF from the adult brains of the housefly Musca domestica was carried out and it was found that an isolated clone (527 bp) encodes a PDF precursor protein of ordinary length. The PDF peptide shows a high sequence identity (78%-94%) and similarity (89%-100%) to insect PDFs and also to the crustacean β-PDH peptides. In particular, there is only a single amino acid difference between the PDFs of Musca and Drosophila; at position 14 Ser for Musca PDF and Asn for Drosophila PDF. A characteristic Ser10 in Drosophila was retained in Musca, indicating the presence of a structural profile unique to these PDFs. The results of sequence analyses suggest that Musca and Drosophila PDFs are to be considered members of a single group that has evolved structurally. When the primary structure of the PAP regions was compared, the Musca PDF precursor also belonged to the same group as that to which the Drosophila PDF precursor belongs.

Original languageEnglish
Pages (from-to)82-91
Number of pages10
JournalJournal of Peptide Science
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 1 2004

Fingerprint

Houseflies
Protein Precursors
Cloning
Neuropeptides
Pigments
Insects
Organism Cloning
Complementary DNA
Peptides
Drosophila
Gryllidae
Amino Acids
Nuclear Localization Signals
Hemiptera
Circadian Clocks
Locomotion
Protein Sorting Signals
Circadian Rhythm
Diptera
Neurotransmitter Agents

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

Cite this

cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptides comparison among the insect circadian neuropeptides. / Matsushima, Ayami; Sato, Seiji; Chuman, Yoshiro; Takeda, Yukimasa; Yokotani, Satoru; Nose, Takeru; Tominaga, Yoshiya; Shimohigashi, Miki; Shimohigashi, Yasuyuki.

In: Journal of Peptide Science, Vol. 10, No. 2, 01.02.2004, p. 82-91.

Research output: Contribution to journalArticle

Matsushima, Ayami ; Sato, Seiji ; Chuman, Yoshiro ; Takeda, Yukimasa ; Yokotani, Satoru ; Nose, Takeru ; Tominaga, Yoshiya ; Shimohigashi, Miki ; Shimohigashi, Yasuyuki. / cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptides comparison among the insect circadian neuropeptides. In: Journal of Peptide Science. 2004 ; Vol. 10, No. 2. pp. 82-91.
@article{67fb1366c0334f6bab52babde3a0f970,
title = "cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptides comparison among the insect circadian neuropeptides",
abstract = "Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that of the cricket Gryllus bimaculatus and that of the last-summer cicada Meimuna opalifera. The G. bimaculatus PDF precursor is extraordinarily short and comprises a nuclear localization signal (NLS), while the M. opalifera PDF precursor is of ordinary length, comparable to that seen for the precursors of crustacean β-PDH homologues. Although their PDF peptide regions were exactly the same, the regions containing a signal peptide combined with a PDF-associated peptide (PAP) were remarkably different from each other. Such a grouping suggested a fundamental role for the PAP peptide in the circadian clock, perhaps associated with PDF function. In the present study, the cDNA cloning of PDF from the adult brains of the housefly Musca domestica was carried out and it was found that an isolated clone (527 bp) encodes a PDF precursor protein of ordinary length. The PDF peptide shows a high sequence identity (78{\%}-94{\%}) and similarity (89{\%}-100{\%}) to insect PDFs and also to the crustacean β-PDH peptides. In particular, there is only a single amino acid difference between the PDFs of Musca and Drosophila; at position 14 Ser for Musca PDF and Asn for Drosophila PDF. A characteristic Ser10 in Drosophila was retained in Musca, indicating the presence of a structural profile unique to these PDFs. The results of sequence analyses suggest that Musca and Drosophila PDFs are to be considered members of a single group that has evolved structurally. When the primary structure of the PAP regions was compared, the Musca PDF precursor also belonged to the same group as that to which the Drosophila PDF precursor belongs.",
author = "Ayami Matsushima and Seiji Sato and Yoshiro Chuman and Yukimasa Takeda and Satoru Yokotani and Takeru Nose and Yoshiya Tominaga and Miki Shimohigashi and Yasuyuki Shimohigashi",
year = "2004",
month = "2",
day = "1",
doi = "10.1002/psc.511",
language = "English",
volume = "10",
pages = "82--91",
journal = "Journal of Peptide Science",
issn = "1075-2617",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - cDNA cloning of the housefly pigment-dispersing factor (PDF) precursor protein and its peptides comparison among the insect circadian neuropeptides

AU - Matsushima, Ayami

AU - Sato, Seiji

AU - Chuman, Yoshiro

AU - Takeda, Yukimasa

AU - Yokotani, Satoru

AU - Nose, Takeru

AU - Tominaga, Yoshiya

AU - Shimohigashi, Miki

AU - Shimohigashi, Yasuyuki

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that of the cricket Gryllus bimaculatus and that of the last-summer cicada Meimuna opalifera. The G. bimaculatus PDF precursor is extraordinarily short and comprises a nuclear localization signal (NLS), while the M. opalifera PDF precursor is of ordinary length, comparable to that seen for the precursors of crustacean β-PDH homologues. Although their PDF peptide regions were exactly the same, the regions containing a signal peptide combined with a PDF-associated peptide (PAP) were remarkably different from each other. Such a grouping suggested a fundamental role for the PAP peptide in the circadian clock, perhaps associated with PDF function. In the present study, the cDNA cloning of PDF from the adult brains of the housefly Musca domestica was carried out and it was found that an isolated clone (527 bp) encodes a PDF precursor protein of ordinary length. The PDF peptide shows a high sequence identity (78%-94%) and similarity (89%-100%) to insect PDFs and also to the crustacean β-PDH peptides. In particular, there is only a single amino acid difference between the PDFs of Musca and Drosophila; at position 14 Ser for Musca PDF and Asn for Drosophila PDF. A characteristic Ser10 in Drosophila was retained in Musca, indicating the presence of a structural profile unique to these PDFs. The results of sequence analyses suggest that Musca and Drosophila PDFs are to be considered members of a single group that has evolved structurally. When the primary structure of the PAP regions was compared, the Musca PDF precursor also belonged to the same group as that to which the Drosophila PDF precursor belongs.

AB - Pigment-dispersing factor (PDF), an 18-amino acid neuropeptide, is a principal circadian neurotransmitter for the circadian rhythms of the locomotor activity in flies. Recently, two completely different types of PDF precursor were clarified; that of the cricket Gryllus bimaculatus and that of the last-summer cicada Meimuna opalifera. The G. bimaculatus PDF precursor is extraordinarily short and comprises a nuclear localization signal (NLS), while the M. opalifera PDF precursor is of ordinary length, comparable to that seen for the precursors of crustacean β-PDH homologues. Although their PDF peptide regions were exactly the same, the regions containing a signal peptide combined with a PDF-associated peptide (PAP) were remarkably different from each other. Such a grouping suggested a fundamental role for the PAP peptide in the circadian clock, perhaps associated with PDF function. In the present study, the cDNA cloning of PDF from the adult brains of the housefly Musca domestica was carried out and it was found that an isolated clone (527 bp) encodes a PDF precursor protein of ordinary length. The PDF peptide shows a high sequence identity (78%-94%) and similarity (89%-100%) to insect PDFs and also to the crustacean β-PDH peptides. In particular, there is only a single amino acid difference between the PDFs of Musca and Drosophila; at position 14 Ser for Musca PDF and Asn for Drosophila PDF. A characteristic Ser10 in Drosophila was retained in Musca, indicating the presence of a structural profile unique to these PDFs. The results of sequence analyses suggest that Musca and Drosophila PDFs are to be considered members of a single group that has evolved structurally. When the primary structure of the PAP regions was compared, the Musca PDF precursor also belonged to the same group as that to which the Drosophila PDF precursor belongs.

UR - http://www.scopus.com/inward/record.url?scp=1342283269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342283269&partnerID=8YFLogxK

U2 - 10.1002/psc.511

DO - 10.1002/psc.511

M3 - Article

C2 - 14994986

AN - SCOPUS:1342283269

VL - 10

SP - 82

EP - 91

JO - Journal of Peptide Science

JF - Journal of Peptide Science

SN - 1075-2617

IS - 2

ER -