CDw90 (Thy-1) expression on CD34 positive cells in peripheral blood stem cell harvest

K. Miyamoto, T. Otsuka, Shinichi Mizuno, K. Funatsu, R. Inaba

Research output: Contribution to journalArticle

Abstract

In this study, we investigated CDw90 (Thy-1) expression in CD34+ cells and hematopoietic colony-forming ability in purified CD34+ Thy-1+ and CD34+ Thy-1 fractions in the peripheral blood at the PBSCH. The high proliferative potential colony-forming cells (HPP-CFC), which are considered to be more primitive hematopoietic stem cells, were found more frequently in the purified CD34+ Thy-1+ fraction than in the purified CD34+ Thy-1- fraction. Thus, it is useful to decide the optimal time of PBSCH to measure the CD34+ Thy-1+ cells as well as CD34+ cells in the peripheral blood by flow-cytometry. In CD34+ acute myeloblastic leukemia (AML) cells, the co-expression rate of CD34 and Thy-1 was negative, suggesting that it may be possible to estimate the contamination of CD34+ AML cells by the Thy-1 expression rate in CD34+ cells at PBSCH.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
Journal[Rinshō ketsueki] The Japanese journal of clinical hematology
Volume38
Issue number1
Publication statusPublished - Jan 1 1997

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Acute Myeloid Leukemia
Hematopoietic Stem Cells
Peripheral Blood Stem Cells
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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CDw90 (Thy-1) expression on CD34 positive cells in peripheral blood stem cell harvest. / Miyamoto, K.; Otsuka, T.; Mizuno, Shinichi; Funatsu, K.; Inaba, R.

In: [Rinshō ketsueki] The Japanese journal of clinical hematology, Vol. 38, No. 1, 01.01.1997, p. 9-13.

Research output: Contribution to journalArticle

Miyamoto, K. ; Otsuka, T. ; Mizuno, Shinichi ; Funatsu, K. ; Inaba, R. / CDw90 (Thy-1) expression on CD34 positive cells in peripheral blood stem cell harvest. In: [Rinshō ketsueki] The Japanese journal of clinical hematology. 1997 ; Vol. 38, No. 1. pp. 9-13.
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