CEACAM2-L on Spermatids Interacts with Poliovirus Receptor on Sertoli Cells in Mouse Seminiferous Epithelium

Elsaid Salaheldeen, Ali Howida, Tomohiko Wakayama, Hiroshi Iida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The removal of excess cytoplasm from elongated spermatids by Sertoli cells is the last essential step in spermatogenesis. It requires cell-to-cell recognition between a Sertoli cell and an elongating spermatid through protein-protein interactions. CEACAM2-L, an adhesion molecule of the immunoglobulin superfamily (IgSF), is present at the plasma membrane covering the excess cytoplasm of elongated spermatids, and is possibly involved in the cell-to-cell recognition. In this study, we investigated the interaction between CEACAM2-L and Poliovirus receptor (PVR), which is also from the IgSF and is expressed by Sertoli cells. Immunohistochemical analysis showed that CEACAM2-L expressed on elongated spermatids was in close contact with PVR-positive cell processes of Sertoli cells. Immunoprecipitation experiments both in vivo and in vitro demonstrated a direct heterophilic interaction between CEACAM2-L and PVR. We show that the N-terminal Ig domain of CEACAM2-L was critical for its interaction with PVR. In addition, we found that CEACAM2-L formed heterophilic trans-tetramers with PVR in transfected COS-7 cells. From these data, we propose that Sertoli cells recognize the excess cytoplasm of elongated spermatids through the PVR-CEACAM2-L interaction in mouse testis.

Original languageEnglish
Pages (from-to)632-644
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume62
Issue number9
DOIs
Publication statusPublished - Sep 27 2014

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Seminiferous Epithelium
Spermatids
Sertoli Cells
Cytoplasm
Immunoglobulins
COS Cells
Spermatogenesis
poliovirus receptor
CD66 antigens
Immunoprecipitation
Testis
Proteins
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

CEACAM2-L on Spermatids Interacts with Poliovirus Receptor on Sertoli Cells in Mouse Seminiferous Epithelium. / Salaheldeen, Elsaid; Howida, Ali; Wakayama, Tomohiko; Iida, Hiroshi.

In: Journal of Histochemistry and Cytochemistry, Vol. 62, No. 9, 27.09.2014, p. 632-644.

Research output: Contribution to journalArticle

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