C/EBPΑ is required for development of dendritic cell progenitors

Robert S. Welner, Deepak Bararia, Giovanni Amabile, Akos Czibere, Touati Benoukraf, Christian Bach, K. Disnika Senali Abayratna Wansa, Min Ye, Hong Zhang, Tadafumi Iino, Christopher J. Hetherington, Koichi Akashi, Daniel G. Tenen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Dendritic cells (DCs) are master regulators of the immune system, but molecular regulation of early DC differentiation has been poorly understood. Here, we report that the transcription factor C/EBPa coordinates the development of progenitor cells required for production of multiple categories of DCs. C/EBPa was needed for differentiation from stem/progenitor cells to common DC progenitors (CDPs), but not for transition of CDP to mature DCs. C/EBPa deletion in mature DCs did not affect their numbers or function, suggesting that this transcription factor is not needed for maintenance of DCs in lymphoid tissues. ChIP-seq and microarrays were used to identify candidate genes regulated by C/EBPa and required for DC formation. Genes previously shown to be critical for DC formation were bound by C/EBPa, and their expression was decreased in the earliest hematopoietic compartments in the absence of C/EBPa. These data indicate that C/EBPa is important for the earliest stages of steady-state DC differentiation.

Original languageEnglish
Pages (from-to)4073-4081
Number of pages9
JournalBlood
Volume121
Issue number20
DOIs
Publication statusPublished - May 16 2013

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Dendritic Cells
Stem Cells
Cell Differentiation
Transcription Factors
Genes
Immune system
Lymphoid Tissue
Microarrays
Stem cells
Immune System
Maintenance
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Welner, R. S., Bararia, D., Amabile, G., Czibere, A., Benoukraf, T., Bach, C., ... Tenen, D. G. (2013). C/EBPΑ is required for development of dendritic cell progenitors. Blood, 121(20), 4073-4081. https://doi.org/10.1182/blood-2012-10-463448

C/EBPΑ is required for development of dendritic cell progenitors. / Welner, Robert S.; Bararia, Deepak; Amabile, Giovanni; Czibere, Akos; Benoukraf, Touati; Bach, Christian; Wansa, K. Disnika Senali Abayratna; Ye, Min; Zhang, Hong; Iino, Tadafumi; Hetherington, Christopher J.; Akashi, Koichi; Tenen, Daniel G.

In: Blood, Vol. 121, No. 20, 16.05.2013, p. 4073-4081.

Research output: Contribution to journalArticle

Welner, RS, Bararia, D, Amabile, G, Czibere, A, Benoukraf, T, Bach, C, Wansa, KDSA, Ye, M, Zhang, H, Iino, T, Hetherington, CJ, Akashi, K & Tenen, DG 2013, 'C/EBPΑ is required for development of dendritic cell progenitors', Blood, vol. 121, no. 20, pp. 4073-4081. https://doi.org/10.1182/blood-2012-10-463448
Welner RS, Bararia D, Amabile G, Czibere A, Benoukraf T, Bach C et al. C/EBPΑ is required for development of dendritic cell progenitors. Blood. 2013 May 16;121(20):4073-4081. https://doi.org/10.1182/blood-2012-10-463448
Welner, Robert S. ; Bararia, Deepak ; Amabile, Giovanni ; Czibere, Akos ; Benoukraf, Touati ; Bach, Christian ; Wansa, K. Disnika Senali Abayratna ; Ye, Min ; Zhang, Hong ; Iino, Tadafumi ; Hetherington, Christopher J. ; Akashi, Koichi ; Tenen, Daniel G. / C/EBPΑ is required for development of dendritic cell progenitors. In: Blood. 2013 ; Vol. 121, No. 20. pp. 4073-4081.
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