C/EBPβ is required for 'emergency' granulopoiesis

Hideyo Hirai; Pu Zhang; Tajhal Dayaram; Christopher J. Hetherington; Shin Ichi Mizuno; Jiro Imanishi; Koichi Akashi; Daniel G. Tenen

doi:10.1038/ni1354

C/EBPβ is required for 'emergency' granulopoiesis

Hideyo Hirai, Pu Zhang, Tajhal Dayaram, Christopher J. Hetherington, Shin Ichi Mizuno, Jiro Imanishi, Koichi Akashi, Daniel G. Tenen

Internal Medicine

Research output: Contribution to journal › Article › peer-review

295 Citations (Scopus)

Abstract

During 'emergency' situations such as infections, host defense requires rapid mobilization of bone marrow granulocyte progenitors. 'Steady-state' granulopoiesis is absolutely dependent on the C/EBPα transcription factor, but the transcriptional mechanisms underlying emergency granulopoiesis remain unclear. Here we show that large numbers of granulocytes were generated from C/EBPα-deficient progenitors after cytokine stimulation in vivo. Cytokine treatment or fungal infection induced upregulation of C/EBPβ but not C/EBPα or C/EBPε transcripts in granulocyte progenitors, and C/EBPβ-deficient progenitors showed decreased emergency-induced granulopoiesis in vitro and in vivo. C/EBPβ inhibited proliferation less severely than did C/EBPα. These data suggest a critical function for C/EBPβ in emergency granulopoiesis, which demands both differentiation and proliferation of granulocyte precursors.

Original language	English
Pages (from-to)	732-739
Number of pages	8
Journal	Nature Immunology
Volume	7
Issue number	7
DOIs	https://doi.org/10.1038/ni1354
Publication status	Published - Jul 2006

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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10.1038/ni1354

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title = "C/EBPβ is required for 'emergency' granulopoiesis",

abstract = "During 'emergency' situations such as infections, host defense requires rapid mobilization of bone marrow granulocyte progenitors. 'Steady-state' granulopoiesis is absolutely dependent on the C/EBPα transcription factor, but the transcriptional mechanisms underlying emergency granulopoiesis remain unclear. Here we show that large numbers of granulocytes were generated from C/EBPα-deficient progenitors after cytokine stimulation in vivo. Cytokine treatment or fungal infection induced upregulation of C/EBPβ but not C/EBPα or C/EBPε transcripts in granulocyte progenitors, and C/EBPβ-deficient progenitors showed decreased emergency-induced granulopoiesis in vitro and in vivo. C/EBPβ inhibited proliferation less severely than did C/EBPα. These data suggest a critical function for C/EBPβ in emergency granulopoiesis, which demands both differentiation and proliferation of granulocyte precursors.",

author = "Hideyo Hirai and Pu Zhang and Tajhal Dayaram and Hetherington, {Christopher J.} and Mizuno, {Shin Ichi} and Jiro Imanishi and Koichi Akashi and Tenen, {Daniel G.}",

note = "Funding Information: D.A. Gonzalez, H. Iwasaki and all other members of the laboratories of D.G.T. and K.A. for discussions and suggestions. Supported by the National Institutes of Health (HL56745 to D.G.T.).",

year = "2006",

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doi = "10.1038/ni1354",

language = "English",

volume = "7",

pages = "732--739",

journal = "Nature Immunology",

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AU - Hirai, Hideyo

AU - Zhang, Pu

AU - Dayaram, Tajhal

AU - Hetherington, Christopher J.

AU - Mizuno, Shin Ichi

AU - Imanishi, Jiro

AU - Akashi, Koichi

AU - Tenen, Daniel G.

N1 - Funding Information: D.A. Gonzalez, H. Iwasaki and all other members of the laboratories of D.G.T. and K.A. for discussions and suggestions. Supported by the National Institutes of Health (HL56745 to D.G.T.).

PY - 2006/7

Y1 - 2006/7

N2 - During 'emergency' situations such as infections, host defense requires rapid mobilization of bone marrow granulocyte progenitors. 'Steady-state' granulopoiesis is absolutely dependent on the C/EBPα transcription factor, but the transcriptional mechanisms underlying emergency granulopoiesis remain unclear. Here we show that large numbers of granulocytes were generated from C/EBPα-deficient progenitors after cytokine stimulation in vivo. Cytokine treatment or fungal infection induced upregulation of C/EBPβ but not C/EBPα or C/EBPε transcripts in granulocyte progenitors, and C/EBPβ-deficient progenitors showed decreased emergency-induced granulopoiesis in vitro and in vivo. C/EBPβ inhibited proliferation less severely than did C/EBPα. These data suggest a critical function for C/EBPβ in emergency granulopoiesis, which demands both differentiation and proliferation of granulocyte precursors.

AB - During 'emergency' situations such as infections, host defense requires rapid mobilization of bone marrow granulocyte progenitors. 'Steady-state' granulopoiesis is absolutely dependent on the C/EBPα transcription factor, but the transcriptional mechanisms underlying emergency granulopoiesis remain unclear. Here we show that large numbers of granulocytes were generated from C/EBPα-deficient progenitors after cytokine stimulation in vivo. Cytokine treatment or fungal infection induced upregulation of C/EBPβ but not C/EBPα or C/EBPε transcripts in granulocyte progenitors, and C/EBPβ-deficient progenitors showed decreased emergency-induced granulopoiesis in vitro and in vivo. C/EBPβ inhibited proliferation less severely than did C/EBPα. These data suggest a critical function for C/EBPβ in emergency granulopoiesis, which demands both differentiation and proliferation of granulocyte precursors.

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JO - Nature Immunology

JF - Nature Immunology

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ER -

C/EBPβ is required for 'emergency' granulopoiesis

Abstract

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