C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis

Sakiko Satake, Hideyo Hirai, Yoshihiro Hayashi, Nobuaki Shime, Akihiro Tamura, Hisayuki Yao, Satoshi Yoshioka, Yasuo Miura, Tohru Inaba, Naohisa Fujita, Eishi Ashihara, Jiro Imanishi, Teiji Sawa, Taira Maekawa

Research output: Contribution to journalArticle

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Abstract

Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPβ is required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations (#1-5) according to their levels of c-Kit and Ly-6G expression. After the induction of candidemia, rapid mobilization of mature granulocytes and an increase in early granulocyte precursors accompanied by cell cycle acceleration was followed by a gradual increase in granulocytes originating from the immature populations. Upon infection, C/EBPβ was upregulated at the protein level in all the granulopoietic subpopulations. The rapid increase in immature subpopulations #1 and #2 observed in C/EBPβ knockout mice at 1 d postinfection was attenuated. Candidemia-induced cell cycle acceleration and proliferation of hematopoietic stem/progenitors were also impaired. Taken together, these data suggest that C/EBPβ is involved in the efficient amplification of early granulocyte precursors during candidemia-induced emergency granulopoiesis.

Original languageEnglish
Pages (from-to)4546-4555
Number of pages10
JournalJournal of Immunology
Volume189
Issue number9
DOIs
Publication statusPublished - Nov 1 2012

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CCAAT-Enhancer-Binding Proteins
Candidemia
Granulocytes
Emergencies
Cell Cycle
Granulocyte Precursor Cells
Infection
Knockout Mice
Bone Marrow Cells
Transcription Factors
Cell Proliferation
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis. / Satake, Sakiko; Hirai, Hideyo; Hayashi, Yoshihiro; Shime, Nobuaki; Tamura, Akihiro; Yao, Hisayuki; Yoshioka, Satoshi; Miura, Yasuo; Inaba, Tohru; Fujita, Naohisa; Ashihara, Eishi; Imanishi, Jiro; Sawa, Teiji; Maekawa, Taira.

In: Journal of Immunology, Vol. 189, No. 9, 01.11.2012, p. 4546-4555.

Research output: Contribution to journalArticle

Satake, S, Hirai, H, Hayashi, Y, Shime, N, Tamura, A, Yao, H, Yoshioka, S, Miura, Y, Inaba, T, Fujita, N, Ashihara, E, Imanishi, J, Sawa, T & Maekawa, T 2012, 'C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis', Journal of Immunology, vol. 189, no. 9, pp. 4546-4555. https://doi.org/10.4049/jimmunol.1103007
Satake, Sakiko ; Hirai, Hideyo ; Hayashi, Yoshihiro ; Shime, Nobuaki ; Tamura, Akihiro ; Yao, Hisayuki ; Yoshioka, Satoshi ; Miura, Yasuo ; Inaba, Tohru ; Fujita, Naohisa ; Ashihara, Eishi ; Imanishi, Jiro ; Sawa, Teiji ; Maekawa, Taira. / C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis. In: Journal of Immunology. 2012 ; Vol. 189, No. 9. pp. 4546-4555.
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