CedA is a novel Escherichia coli protein that activates the cell division inhibited by chromosomal DNA over-replication

Tsutomu Katayama, Makoto Takata, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We isolated and characterized a new gene related to the control of cell division regulation in Escherichia coli. At 30°C, the dnaAcos mutant causes over-replication of the chromosome, and colony formation is inhibited. We found that, at this temperature, the dnaAcos cells form filaments; therefore, septum formation is inhibited. This inhibition was independent of SfIA, an inhibitor of the septum-forming protein, FtsZ. To identify factors involved in this pathway of inhibition, we isolated seven multicopy suppressors for the cold-sensitive phenotype of the dnaAcos mutant. One of these proved to be a previously unknown gene, which we named cedA. This gene encoded a 12 kDa protein and resided at 38.9 min on the E. coli genome map. A multicopy supply of the cedA gene to the dnaAcos cells did not repress over-replication of the chromosome but did stimulate cell division of the host, the result being growth of cells with an abnormally elevated chromosomal copy number. Therefore, the expression level of the cedA gene seems to be important for inhibiting cell division of the dnaAcos mutant at 30°C. We propose that over-replication of the chromosome activates a pathway for Inhibiting cell division and that the cedA gene modulates this division control. In the dnaA+ background, cedA also seems to affect cell division.

Original languageEnglish
Pages (from-to)687-697
Number of pages11
JournalMolecular Microbiology
Volume26
Issue number4
Publication statusPublished - 1997

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Escherichia coli Proteins
DNA Replication
Cell Division
Genes
Chromosomes
Escherichia coli
Proteins
Genome
Phenotype
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Microbiology

Cite this

CedA is a novel Escherichia coli protein that activates the cell division inhibited by chromosomal DNA over-replication. / Katayama, Tsutomu; Takata, Makoto; Sekimizu, Kazuhisa.

In: Molecular Microbiology, Vol. 26, No. 4, 1997, p. 687-697.

Research output: Contribution to journalArticle

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