TY - JOUR
T1 - Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells
AU - Fukada, Kazuhiro
AU - Takahashi-Yanaga, Fumi
AU - Sakoguchi-Okada, Naoko
AU - Shiraishi, Fumie
AU - Miwa, Yoshikazu
AU - Morimoto, Sachio
AU - Sasaguri, Toshiyuki
PY - 2007/6/15
Y1 - 2007/6/15
N2 - The effect of celecoxib, a cyclooxygenase-2 selective inhibitor, on a human cervical cancer cell line, HeLa cells, was examined. We found that celecoxib increased DNA ladder formation and the activity of caspase-3, indicating that celecoxib induced apoptosis in HeLa cells. Celecoxib suppressed the expression of an anti-apoptotic protein, survivin, in both protein and mRNA levels. The overexpression of survivin overrode caspase-3 activation induced by celecoxib. Subsequently, we performed luciferase reporter assay with the reporter vector containing human survivin promoter region and electrophoretic mobility shift assay and found that the -75 to -66 bp region relative to the initiating codon played an important role in celecoxib action to suppress survivin promoter activity. Our findings might provide a new insight into the anti-cancer effects of celecoxib.
AB - The effect of celecoxib, a cyclooxygenase-2 selective inhibitor, on a human cervical cancer cell line, HeLa cells, was examined. We found that celecoxib increased DNA ladder formation and the activity of caspase-3, indicating that celecoxib induced apoptosis in HeLa cells. Celecoxib suppressed the expression of an anti-apoptotic protein, survivin, in both protein and mRNA levels. The overexpression of survivin overrode caspase-3 activation induced by celecoxib. Subsequently, we performed luciferase reporter assay with the reporter vector containing human survivin promoter region and electrophoretic mobility shift assay and found that the -75 to -66 bp region relative to the initiating codon played an important role in celecoxib action to suppress survivin promoter activity. Our findings might provide a new insight into the anti-cancer effects of celecoxib.
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U2 - 10.1016/j.bbrc.2007.04.077
DO - 10.1016/j.bbrc.2007.04.077
M3 - Article
C2 - 17466271
AN - SCOPUS:34247886162
VL - 357
SP - 1166
EP - 1171
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -