Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells

Kazuhiro Fukada, Fumi Takahashi-Yanaga, Naoko Sakoguchi-Okada, Fumie Shiraishi, Yoshikazu Miwa, Sachio Morimoto, Toshiyuki Sasaguri

Research output: Contribution to journalArticle

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Abstract

The effect of celecoxib, a cyclooxygenase-2 selective inhibitor, on a human cervical cancer cell line, HeLa cells, was examined. We found that celecoxib increased DNA ladder formation and the activity of caspase-3, indicating that celecoxib induced apoptosis in HeLa cells. Celecoxib suppressed the expression of an anti-apoptotic protein, survivin, in both protein and mRNA levels. The overexpression of survivin overrode caspase-3 activation induced by celecoxib. Subsequently, we performed luciferase reporter assay with the reporter vector containing human survivin promoter region and electrophoretic mobility shift assay and found that the -75 to -66 bp region relative to the initiating codon played an important role in celecoxib action to suppress survivin promoter activity. Our findings might provide a new insight into the anti-cancer effects of celecoxib.

Original languageEnglish
Pages (from-to)1166-1171
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number4
DOIs
Publication statusPublished - Jun 15 2007

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Celecoxib
HeLa Cells
Apoptosis
Caspase 3
Assays
Electrophoretic mobility
Apoptosis Regulatory Proteins
Cyclooxygenase 2 Inhibitors
Ladders
Electrophoretic Mobility Shift Assay
Cyclooxygenase 2
Luciferases
Genetic Promoter Regions
Codon
Uterine Cervical Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells. / Fukada, Kazuhiro; Takahashi-Yanaga, Fumi; Sakoguchi-Okada, Naoko; Shiraishi, Fumie; Miwa, Yoshikazu; Morimoto, Sachio; Sasaguri, Toshiyuki.

In: Biochemical and Biophysical Research Communications, Vol. 357, No. 4, 15.06.2007, p. 1166-1171.

Research output: Contribution to journalArticle

Fukada, K, Takahashi-Yanaga, F, Sakoguchi-Okada, N, Shiraishi, F, Miwa, Y, Morimoto, S & Sasaguri, T 2007, 'Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells', Biochemical and Biophysical Research Communications, vol. 357, no. 4, pp. 1166-1171. https://doi.org/10.1016/j.bbrc.2007.04.077
Fukada K, Takahashi-Yanaga F, Sakoguchi-Okada N, Shiraishi F, Miwa Y, Morimoto S et al. Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells. Biochemical and Biophysical Research Communications. 2007 Jun 15;357(4):1166-1171. https://doi.org/10.1016/j.bbrc.2007.04.077
Fukada, Kazuhiro ; Takahashi-Yanaga, Fumi ; Sakoguchi-Okada, Naoko ; Shiraishi, Fumie ; Miwa, Yoshikazu ; Morimoto, Sachio ; Sasaguri, Toshiyuki. / Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 357, No. 4. pp. 1166-1171.
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