Celecoxib induces apoptosis by inhibiting the expression of survivin in HeLa cells

Kazuhiro Fukada, Fumi Takahashi-Yanaga, Naoko Sakoguchi-Okada, Fumie Shiraishi, Yoshikazu Miwa, Sachio Morimoto, Toshiyuki Sasaguri

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The effect of celecoxib, a cyclooxygenase-2 selective inhibitor, on a human cervical cancer cell line, HeLa cells, was examined. We found that celecoxib increased DNA ladder formation and the activity of caspase-3, indicating that celecoxib induced apoptosis in HeLa cells. Celecoxib suppressed the expression of an anti-apoptotic protein, survivin, in both protein and mRNA levels. The overexpression of survivin overrode caspase-3 activation induced by celecoxib. Subsequently, we performed luciferase reporter assay with the reporter vector containing human survivin promoter region and electrophoretic mobility shift assay and found that the -75 to -66 bp region relative to the initiating codon played an important role in celecoxib action to suppress survivin promoter activity. Our findings might provide a new insight into the anti-cancer effects of celecoxib.

Original languageEnglish
Pages (from-to)1166-1171
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number4
DOIs
Publication statusPublished - Jun 15 2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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