The cell adhesion and migration behavior on different nanopatterned surfaces was investigated to determine the effect of different nano-topography on cell function. The adsorption of extracellular matrix protein like fibronectin, on different nanopatterned surfaces, was performed by using immunofluorescence and contact angle measurements. The initial cell adhesion strength on the different substrates was studied by the use of laser tweezers. The live imaging of cell migration on the different substrates with cells transfected with EYFP-alpha-tubulin vector was studied. The initial cell culture on coated and plain mica strips demonstrated that hMSC displayed more of a polar morphology with long filopodium extensions on worm-like patterned substrates.