Abstract
We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - Dec 1 2005 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 5/25/05 → 5/27/05 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Engineering(all)
Cite this
Cell adhesion on the surface preadsorbed with fibronectin and albumin. / Arima, Yusuke; Horikawa, Tsuyoshi; Iwata, Hiroo.
2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.Research output: Contribution to conference › Paper
}
TY - CONF
T1 - Cell adhesion on the surface preadsorbed with fibronectin and albumin
AU - Arima, Yusuke
AU - Horikawa, Tsuyoshi
AU - Iwata, Hiroo
PY - 2005/12/1
Y1 - 2005/12/1
N2 - We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.
AB - We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.
UR - http://www.scopus.com/inward/record.url?scp=33645644882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645644882&partnerID=8YFLogxK
M3 - Paper
AN - SCOPUS:33645644882
ER -