Cell adhesion on the surface preadsorbed with fibronectin and albumin

Yusuke Arima; Tsuyoshi Horikawa; Hiroo Iwata

Cell adhesion on the surface preadsorbed with fibronectin and albumin

Yusuke Arima, Tsuyoshi Horikawa, Hiroo Iwata

Research output: Contribution to conference › Paper › peer-review

Abstract

We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH ₂ > OH > CH₃. HUVECs adhered on COOH and NH ₂-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.

Original language	English
Number of pages	1
Publication status	Published - Dec 1 2005
Externally published	Yes
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	5/25/05 → 5/27/05

All Science Journal Classification (ASJC) codes

Engineering(all)

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title = "Cell adhesion on the surface preadsorbed with fibronectin and albumin",

abstract = "We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.",

author = "Yusuke Arima and Tsuyoshi Horikawa and Hiroo Iwata",

year = "2005",

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day = "1",

language = "English",

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T1 - Cell adhesion on the surface preadsorbed with fibronectin and albumin

AU - Arima, Yusuke

AU - Horikawa, Tsuyoshi

AU - Iwata, Hiroo

PY - 2005/12/1

Y1 - 2005/12/1

N2 - We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.

AB - We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.

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T2 - 54th SPSJ Annual Meeting 2005

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