Cell adhesion on the surface preadsorbed with fibronectin and albumin

Yusuke Arima, Tsuyoshi Horikawa, Hiroo Iwata

Research output: Contribution to conferencePaper

2 Citations (Scopus)

Abstract

We examined the effect of existence of preadsorbed protein layers on cell adhesion. Mixed solutions of fibronectin (Fn) and albumin (BSA) were preadsorbed on serf-assembled monolayers presenting different functional groups including methyl, hydroxyl, carboxyl, and amine, and then human umbilical vein endothelial cells (HUVECs) were plated on the preadsorbed surfaces. Under the preadsorbed condition of Fn in the presence of more than 100-hold BSA, HUVEC adhesion depended on terminal functional group of the SAM as follows: COOH > NH 2 > OH > CH3. HUVECs adhered on COOH and NH 2-SAM preadsorbed with BSA, whereas HUVECs did not adhere on these surfaces immobilized with BSA. These results suggest that exchange of preadsorbed BSA with cell-adhesive proteins in liquid phase, such as fibronectin and vitronectin, induced cell adhesion on these surfaces.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

Fingerprint

Cell adhesion
Endothelial cells
Functional groups
Proteins
Amines
Monolayers
Adhesives
Liquids

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Arima, Y., Horikawa, T., & Iwata, H. (2005). Cell adhesion on the surface preadsorbed with fibronectin and albumin. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Cell adhesion on the surface preadsorbed with fibronectin and albumin. / Arima, Yusuke; Horikawa, Tsuyoshi; Iwata, Hiroo.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

Arima, Y, Horikawa, T & Iwata, H 2005, 'Cell adhesion on the surface preadsorbed with fibronectin and albumin' Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 5/25/05 - 5/27/05, .
Arima Y, Horikawa T, Iwata H. Cell adhesion on the surface preadsorbed with fibronectin and albumin. 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Arima, Yusuke ; Horikawa, Tsuyoshi ; Iwata, Hiroo. / Cell adhesion on the surface preadsorbed with fibronectin and albumin. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
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