TY - JOUR
T1 - Cell Behavior within Nanogrooved Sandwich Culture Systems
AU - Bjørge, Isabel M.
AU - Salmeron-Sanchez, Manuel
AU - Correia, Clara R.
AU - Mano, João F.
N1 - Funding Information:
I.M.Bj?rge acknowledges financial support by the Portuguese Foundation for Science and Technology (FCT) with doctoral grant SFRH/BD/129224/2017. This work was supported by the European Research Council grant agreement for the project ?ATLAS? (ERC-2014-ADG-669858) and the FCT project ?CIRCUS? (PTDC/BTM-MAT/31064/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. Image acquisition was performed by Dr. A. Sofia Silva in the LiM facility of iBiMED, a node of PPBI (Portuguese 4 Platform of BioImaging): POCI-01-0145-FEDER-022122.
Funding Information:
I.M.Bjørge acknowledges financial support by the Portuguese Foundation for Science and Technology (FCT) with doctoral grant SFRH/BD/129224/2017. This work was supported by the European Research Council grant agreement for the project “ATLAS” (ERC‐2014‐ADG‐669858) and the FCT project “CIRCUS” (PTDC/BTM‐MAT/31064/2017). This work was developed within the scope of the project CICECO‐Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co‐financed by FEDER under the PT2020 Partnership Agreement. Image acquisition was performed by Dr. A. Sofia Silva in the LiM facility of iBiMED, a node of PPBI (Portuguese 4 Platform of BioImaging): POCI‐01‐0145‐FEDER‐022122.
Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Grooved topography and inherent cell contact guidance has shown promising results regarding cell proliferation, morphology, and lineage-specific differentiation. Yet these approaches are limited to 2D applications. Sandwich-culture conditions are developed to bridge the gap between 2D and 3D culture, enabling both ventral and dorsal cell surface stimulation. The effect of grooved surface topography is accessed on cell orientation and elongation in a highly controlled manner, with simultaneous and independent stimuli on two cell sides. Nanogrooved and non-nanogrooved substrates are assembled into quasi-3D systems with variable relative orientations. A plethora of sandwich-culture conditions are created by seeding cells on lower, upper, or both substrates. Software image analysis demonstrates that F-actin of cells acquires the orientation of the substrate on which cells are initially seeded, independently from the orientation of the second top substrate. Contrasting cell morphologies are observed, with a higher elongation for nanogrooved 2D substrates than nanogrooved sandwich-culture conditions. Correlated with an increased pFAK activity and vinculin staining for sandwich-culture conditions, these results point to an enhanced cell surface stimulation versus control conditions. The pivotal role of initial cell-biomaterial contact on cellular alignment is highlighted, providing important insights for tissue engineering strategies aiming to guide cellular response through mechanotransduction approaches.
AB - Grooved topography and inherent cell contact guidance has shown promising results regarding cell proliferation, morphology, and lineage-specific differentiation. Yet these approaches are limited to 2D applications. Sandwich-culture conditions are developed to bridge the gap between 2D and 3D culture, enabling both ventral and dorsal cell surface stimulation. The effect of grooved surface topography is accessed on cell orientation and elongation in a highly controlled manner, with simultaneous and independent stimuli on two cell sides. Nanogrooved and non-nanogrooved substrates are assembled into quasi-3D systems with variable relative orientations. A plethora of sandwich-culture conditions are created by seeding cells on lower, upper, or both substrates. Software image analysis demonstrates that F-actin of cells acquires the orientation of the substrate on which cells are initially seeded, independently from the orientation of the second top substrate. Contrasting cell morphologies are observed, with a higher elongation for nanogrooved 2D substrates than nanogrooved sandwich-culture conditions. Correlated with an increased pFAK activity and vinculin staining for sandwich-culture conditions, these results point to an enhanced cell surface stimulation versus control conditions. The pivotal role of initial cell-biomaterial contact on cellular alignment is highlighted, providing important insights for tissue engineering strategies aiming to guide cellular response through mechanotransduction approaches.
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U2 - 10.1002/smll.202001975
DO - 10.1002/smll.202001975
M3 - Article
C2 - 32603002
AN - SCOPUS:85087220805
VL - 16
JO - Small
JF - Small
SN - 1613-6810
IS - 31
M1 - 2001975
ER -