Cell-cell adhesion in limb-formation, estimated from photographs of cell sorting experiments based on a spatial stochastic model

Atsushi Mochizuki, Naoyuki Wada, Hiroyuki Ide, Yoh Iwasa

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Abstract

We developed a new method to estimate the magnitude of differential cell-cell adhesion of two tissues based on the spatial patterns in cell- sorting experiments, and applied it to experimental data on progress-zone cells of avian limb bud at stages 20-26. The change in cell distribution in the experiment was recorded, and statistics q(B/B) for the degree of cell sorting was calculated from the photographs. Based on extensive computer simulations of spatial Markov processes on a 2-D lattice, we derived a formula for q(B/B) increasing with time. Using least square fitting, differential adhesion A and cell motility m are estimated from the time series data of q(B/B) obtained from the experiment. The estimated A was close to 0 (the spatial pattern remained random) if the mixed cells were from two tissues of the same stage. If the mixed cells were from different stages, and the estimated A was positive (cell sorting occurred). Estimated A increased with the difference in the stage number of the two tissues from which the cells were sampled. This result can be explained both by the stage-specific change in adhesion molecules and by a linear increase (or decrease) in the amount of adhesion molecules on cell surface.

Original languageEnglish
Pages (from-to)204-214
Number of pages11
JournalDevelopmental Dynamics
Volume211
Issue number3
DOIs
Publication statusPublished - Mar 1 1998

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All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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