Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis

Kosuke Ino, Mina Okochi, Nao Konishi, Masahiro Nakatochi, Rentaro Imai, Mitsuhiro Shikida, Akira Ito, Hiroyuki Honda

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

In order to understand the behavior of individual cells, single cell analyses have attracted attention since most cell-based assays provide data with values averaged across a large number of cells. Techniques for the manipulation and analysis of single cells are crucial for understanding the behavior of individual cells. In the present study, we have developed single cell culture arrays using magnetic force and a pin holder, which enables the allocation of the magnetically labeled cells on arrays, and have analyzed their dynamics. The pin holder was made from magnetic soft iron and contained more than 6000 pillars on its surface. The pin holder was placed on a magnet to concentrate the magnetic flux density above the pillars. NIH/3T3 fibroblasts that were labeled with magnetite cationic liposomes (MCLs) were seeded into a culture dish, and the dish was placed over the pin holder with the magnet. The magnetically labeled cells were guided on the surface where the pillars were positioned and allocated on the arrays with a high resolution. Single-cell patterning was achieved by adjusting the number of cells seeded, and the target cell was collected by a micromanipulator after removing the pin holder with the magnet. Furthermore, change in the morphology of magnetically patterned cells was analyzed by microscopic observation, and cell spreading on the array was observed with time duration. Magnetic force-based cell patterning on cell culture arrays would be a suitable technique for the analysis of cell behavior in studies of cell-cell variation and cell-cell interactions.

Original languageEnglish
Pages (from-to)134-142
Number of pages9
JournalLab on a Chip
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

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Single-Cell Analysis
Cell culture
Magnets
Cell Culture Techniques
Cells
Micromanipulators
Ferrosoferric Oxide
Liposomes
Magnetite
Magnetic flux
Fibroblasts
Assays
Iron
Cell Count

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Ino, K., Okochi, M., Konishi, N., Nakatochi, M., Imai, R., Shikida, M., ... Honda, H. (2007). Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis. Lab on a Chip, 8(1), 134-142. https://doi.org/10.1039/b712330b

Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis. / Ino, Kosuke; Okochi, Mina; Konishi, Nao; Nakatochi, Masahiro; Imai, Rentaro; Shikida, Mitsuhiro; Ito, Akira; Honda, Hiroyuki.

In: Lab on a Chip, Vol. 8, No. 1, 01.01.2007, p. 134-142.

Research output: Contribution to journalArticle

Ino, K, Okochi, M, Konishi, N, Nakatochi, M, Imai, R, Shikida, M, Ito, A & Honda, H 2007, 'Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis', Lab on a Chip, vol. 8, no. 1, pp. 134-142. https://doi.org/10.1039/b712330b
Ino K, Okochi M, Konishi N, Nakatochi M, Imai R, Shikida M et al. Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis. Lab on a Chip. 2007 Jan 1;8(1):134-142. https://doi.org/10.1039/b712330b
Ino, Kosuke ; Okochi, Mina ; Konishi, Nao ; Nakatochi, Masahiro ; Imai, Rentaro ; Shikida, Mitsuhiro ; Ito, Akira ; Honda, Hiroyuki. / Cell culture arrays using magnetic force-based cell patterning for dynamic single cell analysis. In: Lab on a Chip. 2007 ; Vol. 8, No. 1. pp. 134-142.
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