Cell cycle arrest induced by radiation in cultured silkworm cells

Masateru Takahashi, Jae Man Lee, Hiroaki Mon, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The silkworm, Bombyx mori, is known to be more resistant to ionizing radiation (IR) compared with mammals, but detailed processes underlying this resistance have remained largely unexplored. In this study, we have confirmed that silkworm larvae survived and showed no effect in the apparent reproduction ability after the irradiation of high doses of γ -ray. We have then observed the effects of γ -irradiation to cells of the silkworm cell line BmN4, which showed marked cell-cycle arrest at the G2/M phase, but not at the G1, or S phases. These cells did not undergo apoptosis after γ -ray irradiation in contrast to the mammalian cells wherein G1, arrests after IR causes apoptosis. After UV-C radiation to cells of BmN4 and Sf21 (a Spodoptera frugiperuda cell line), S-phase checkpoint activation was provoked. But there was also no observation of cell death in BmN4 cells, in contrast to Sf21 cells. Based on these results, we proposed that the extraordinary tolerance of induced DNA injury or the elimination of cell death programs after irradiation is a possible cause of the irradiation resistance in the silkworm cells.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalJournal of Insect Biotechnology and Sericology
Volume75
Issue number1
Publication statusPublished - Feb 1 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Fingerprint Dive into the research topics of 'Cell cycle arrest induced by radiation in cultured silkworm cells'. Together they form a unique fingerprint.

Cite this