Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells

Mayumi Okamoto, Takaki Miyata, Daijiro Konno, Hiroki R. Ueda, Takeya Kasukawa, Mitsuhiro Hashimoto, Fumio Matsuzaki, Ayano Kawaguchi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

During cerebral development, many types of neurons are sequentially generated by self-renewing progenitor cells called apical progenitors (APs). Temporal changes in AP identity are thought to be responsible for neuronal diversity; however, the mechanisms underlying such changes remain largely unknown. Here we perform single-cell transcriptome analysis of individual progenitors at different developmental stages, and identify a subset of genes whose expression changes over time but is independent of differentiation status. Surprisingly, the pattern of changes in the expression of such temporal-axis genes in APs is unaffected by cell-cycle arrest. Consistent with this, transient cell-cycle arrest of APs in vivo does not prevent descendant neurons from acquiring their correct laminar fates. Analysis of cultured APs reveals that transitions in AP gene expression are driven by both cell-intrinsic and-extrinsic mechanisms. These results suggest that the timing mechanisms controlling AP temporal identity function independently of cell-cycle progression and Notch activation mode.

Original languageEnglish
Article number11349
JournalNature communications
Volume7
DOIs
Publication statusPublished - Apr 20 2016

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Cell Cycle
Stem Cells
gene expression
Cells
neurons
Gene expression
cycles
Neurons
Cell Cycle Checkpoints
cells
notches
Single-Cell Analysis
Gene Expression
progressions
genes
set theory
Gene Expression Profiling
Genes
Chemical activation
time measurement

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Okamoto, M., Miyata, T., Konno, D., Ueda, H. R., Kasukawa, T., Hashimoto, M., ... Kawaguchi, A. (2016). Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells. Nature communications, 7, [11349]. https://doi.org/10.1038/ncomms11349

Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells. / Okamoto, Mayumi; Miyata, Takaki; Konno, Daijiro; Ueda, Hiroki R.; Kasukawa, Takeya; Hashimoto, Mitsuhiro; Matsuzaki, Fumio; Kawaguchi, Ayano.

In: Nature communications, Vol. 7, 11349, 20.04.2016.

Research output: Contribution to journalArticle

Okamoto, M, Miyata, T, Konno, D, Ueda, HR, Kasukawa, T, Hashimoto, M, Matsuzaki, F & Kawaguchi, A 2016, 'Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells', Nature communications, vol. 7, 11349. https://doi.org/10.1038/ncomms11349
Okamoto, Mayumi ; Miyata, Takaki ; Konno, Daijiro ; Ueda, Hiroki R. ; Kasukawa, Takeya ; Hashimoto, Mitsuhiro ; Matsuzaki, Fumio ; Kawaguchi, Ayano. / Cell-cycle-independent transitions in temporal identity of mammalian neural progenitor cells. In: Nature communications. 2016 ; Vol. 7.
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