Abstract
Plk (polo-like kinase) is a serine-threonine kinase that appears to function in mitotic control in mammalian cells. We demonstrated previously that PLK mRNA expression is low at the G1-S transition, increases during S phase, and is maximally expressed during G2-M. In the present study, we have cloned the human PLK gene and analyzed the structure and function of 2 kilobases of its 5'-flanking region. Using synchronized cultures of HeLa cells transfected with PLK promoter/luciferase constructs, we show that the promoter of PLK is activated at S phase and is maximal at G2-M phase. Using various PLK promoter/luciferase constructs, we show that three activating regions are located between 35 and 93 base pairs upstream of the transcription initiation site. We identified a repressor element (CDE/CHR) in the region of the transcription start site, and mutations within this element diminished cell cycle regulation of transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 9166-9174 |
| Number of pages | 9 |
| Journal | Journal of Biological Chemistry |
| Volume | 272 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - Apr 4 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology
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Cell cycle regulation of the human polo-like kinase (PLK) promoter. / Uchiumi, Takeshi; Longo, Dan L.; Ferris, Douglas K.
In: Journal of Biological Chemistry, Vol. 272, No. 14, 04.04.1997, p. 9166-9174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell cycle regulation of the human polo-like kinase (PLK) promoter
AU - Uchiumi, Takeshi
AU - Longo, Dan L.
AU - Ferris, Douglas K.
PY - 1997/4/4
Y1 - 1997/4/4
N2 - Plk (polo-like kinase) is a serine-threonine kinase that appears to function in mitotic control in mammalian cells. We demonstrated previously that PLK mRNA expression is low at the G1-S transition, increases during S phase, and is maximally expressed during G2-M. In the present study, we have cloned the human PLK gene and analyzed the structure and function of 2 kilobases of its 5'-flanking region. Using synchronized cultures of HeLa cells transfected with PLK promoter/luciferase constructs, we show that the promoter of PLK is activated at S phase and is maximal at G2-M phase. Using various PLK promoter/luciferase constructs, we show that three activating regions are located between 35 and 93 base pairs upstream of the transcription initiation site. We identified a repressor element (CDE/CHR) in the region of the transcription start site, and mutations within this element diminished cell cycle regulation of transcription.
AB - Plk (polo-like kinase) is a serine-threonine kinase that appears to function in mitotic control in mammalian cells. We demonstrated previously that PLK mRNA expression is low at the G1-S transition, increases during S phase, and is maximally expressed during G2-M. In the present study, we have cloned the human PLK gene and analyzed the structure and function of 2 kilobases of its 5'-flanking region. Using synchronized cultures of HeLa cells transfected with PLK promoter/luciferase constructs, we show that the promoter of PLK is activated at S phase and is maximal at G2-M phase. Using various PLK promoter/luciferase constructs, we show that three activating regions are located between 35 and 93 base pairs upstream of the transcription initiation site. We identified a repressor element (CDE/CHR) in the region of the transcription start site, and mutations within this element diminished cell cycle regulation of transcription.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031004775&partnerID=8YFLogxK
U2 - 10.1074/jbc.272.14.9166
DO - 10.1074/jbc.272.14.9166
M3 - Article
C2 - 9083047
AN - SCOPUS:0031004775
VL - 272
SP - 9166
EP - 9174
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 14
ER -