Cell density-dependent regulation of hepatic development by a gp130-independent pathway

Nobuhiko Kojima, Taisei Kinoshita, Akihide Kamiya, Koji Nakamura, Kinichi Nakashima, Tetsuya Taga, Atsushi Miyajima

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44 Citations (Scopus)

Abstract

We previously demonstrated that oncostatin M (OSM) promotes hepatic development in concert with glucocorticoid. The livers from mice deficient for gp130, a signaling subunit of the OSM receptor, displayed reduced expression of hepatic differentiation marker and defective glycogenic function. However, these phenotypes were not completely abolished in gp130(-/-) mice, suggesting that there is an alternative pathway regulating hepatic development in vivo. To test this possibility, we cultured gp130(-/-) fetal hepatic cells and investigated a signal that induces hepatic differentiation. When hepatecytes were forced to interact with each other by inoculating cells at high densities, hepatic differentiation was induced even in the absence of gp130. Moreover, cells stimulated with OSM and/or cultured at a high density possess many other metabolic functions. These observations suggest that fetal hepatic cells acquire multiple characteristics of differentiated hepatocytes in response to the signals generated by cell-cell contacts as well as by OSM. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume277
Issue number1
DOIs
Publication statusPublished - Oct 14 2000

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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