Cell fusion through a microslit between adhered cells and observation of their nuclear behavior

Ken Ichi Wada, Kazuo Hosokawa, Eitaro Kondo, Yoshihiro Ito, Mizuo Maeda

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

This paper describes a novel cell fusion method which induces cell fusion between adhered cells through a microslit for preventing nuclear mixing. For this purpose, a microfluidic device which had ∼100 cell pairing structures (CPSs) making cell pairs through microslits with 2.1±0.3μm width was fabricated. After trapping NIH3T3 cells with hydrodynamic forces at the CPSs, the cells were fused through the microslit by the Sendai virus envelope method. With following timelapse observation, we discovered that the spread cells were much less susceptible to nuclear migration passing through the microslit compared with round cells, and that cytoplasmic fraction containing mitochondria was transferred through the microslit without nuclear mixing. These findings will provide an effective method for cell fusion without nuclear mixing, and will lead to an efficient method for reprograming and transdifferentiation of target cells toward regenerative medicine.

Original languageEnglish
Pages (from-to)1464-1468
Number of pages5
JournalBiotechnology and Bioengineering
Volume111
Issue number7
DOIs
Publication statusPublished - Jul 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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