Cell Kinetics of Intracerebrally Implanted RG-C6 Tumors in Rats: Effects of Intracarotid Hyperosmolar Mannitol measured by 3H-thymidine Autoradiography and Bromodeoxyuridine Uptake

Tooru Inoue, Masashi Fukui, Iwao Takeshita, Toru Iwaki

Research output: Contribution to journalArticle

Abstract

The proliferative activity of intracerebrally implanted rat glioma C6 (RG-C6) tumors in rats was exam ined by means of 3H-thymidine autoradiography and measurement of bromodeoxyuridine (BrdU) uptake. The effect of intracarotid hyperosmolar mannitol on the cell kinetics of the RG-C6 tumors was studied by the latter method. The labeling index (LI) and distribution of 3H-thymidine-labeled cells were identical to those of the BrdU-labeled cells. The LIs of BrdU-labeled cells were not altered by in tracarotid mannitol injection. The influence of intracarotid hyperosmolar mannitol infusion not only on brain tumors but also on normal brain tissue is discussed.

Original languageEnglish
Pages (from-to)533-537
Number of pages5
JournalNeurologia medico-chirurgica
Volume28
Issue number6
DOIs
Publication statusPublished - Jan 1 1988

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Mannitol
Bromodeoxyuridine
Autoradiography
Glioma
Thymidine
Neoplasms
Brain Neoplasms
Injections
Brain

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cite this

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title = "Cell Kinetics of Intracerebrally Implanted RG-C6 Tumors in Rats: Effects of Intracarotid Hyperosmolar Mannitol measured by 3H-thymidine Autoradiography and Bromodeoxyuridine Uptake",
abstract = "The proliferative activity of intracerebrally implanted rat glioma C6 (RG-C6) tumors in rats was exam ined by means of 3H-thymidine autoradiography and measurement of bromodeoxyuridine (BrdU) uptake. The effect of intracarotid hyperosmolar mannitol on the cell kinetics of the RG-C6 tumors was studied by the latter method. The labeling index (LI) and distribution of 3H-thymidine-labeled cells were identical to those of the BrdU-labeled cells. The LIs of BrdU-labeled cells were not altered by in tracarotid mannitol injection. The influence of intracarotid hyperosmolar mannitol infusion not only on brain tumors but also on normal brain tissue is discussed.",
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AU - Iwaki, Toru

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AB - The proliferative activity of intracerebrally implanted rat glioma C6 (RG-C6) tumors in rats was exam ined by means of 3H-thymidine autoradiography and measurement of bromodeoxyuridine (BrdU) uptake. The effect of intracarotid hyperosmolar mannitol on the cell kinetics of the RG-C6 tumors was studied by the latter method. The labeling index (LI) and distribution of 3H-thymidine-labeled cells were identical to those of the BrdU-labeled cells. The LIs of BrdU-labeled cells were not altered by in tracarotid mannitol injection. The influence of intracarotid hyperosmolar mannitol infusion not only on brain tumors but also on normal brain tissue is discussed.

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