In this chapter, we propose “on-chip robotics” that is based on robot technologies and lab-on-a-chip devices to achieve high-speed, high-accuracy, and quantitative cell manipulation and analysis on a chip. We presents a high-throughput cell mechanical characterization method using a robot integrated microfluidic chip (robochip). The robochip contains a magnetically driven on-chip probe, a force sensor, and microchannels. The displacement reduction mechanism is adopted to the on-chip probe for high resolution positioning of the tip of the probe, and we achieved noncontact actuation of the on-chip probe with 0.18 μm in repetitive positioning. We show the automated mechanical characterization of oocyte. The throughput of the measurement is approximately 15–20 s per oocyte, and we conclude that high-throughput cellular mechanical characterization is achieved. We also show measurements of the viscoelastic properties of oocyte using the robochip. The proposed approach based on a robochip is a promising technology for contributing to the analysis of the physical and biological properties of cells.
|Title of host publication||Hyper Bio Assembler for 3D Cellular Systems|
|Number of pages||20|
|Publication status||Published - Jan 1 2015|
All Science Journal Classification (ASJC) codes
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)