Cell patterning on polylactic acid through surface-tethered oligonucleotides

Toshiki Matsui, Yusuke Arima, Naohiro Takemoto, Hiroo Iwata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Polylactic acid (PLA) is a candidate material to prepare scaffolds for 3-D tissue regeneration. However, cells do not adhere or proliferate well on the surface of PLA because it is hydrophobic. We report a simple and rapid method for inducing cell adhesion to PLA through DNA hybridization. Single-stranded DNA (ssDNA) conjugated to poly(ethylene glycol) (PEG) and to a terminal phospholipid (ssDNA-PEG-lipid) was used for cell surface modification. Through DNA hybridization, modified cells were able to attach to PLA surfaces modified with complementary sequence (ssDNA′). Different cell types can be attached to PLA fibers and films in a spatially controlled manner by using ssDNAs with different sequences. In addition, they proliferate well in a culture medium supplemented with fetal bovine serum. The coexisting modes of cell adhesion through DNA hybridization and natural cytoskeletal adhesion machinery revealed no serious effects on cell growth. The combination of a 3-D scaffold made of PLA and cell immobilization on the PLA scaffold through DNA hybridization will be useful for the preparation of 3-D tissue and organs.

Original languageEnglish
Pages (from-to)32-41
Number of pages10
JournalActa Biomaterialia
Volume13
DOIs
Publication statusPublished - Feb 1 2015

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Oligonucleotides
DNA
Acids
Single-Stranded DNA
Polyethylene glycols
Cell adhesion
Scaffolds (biology)
Cell Adhesion
Cell immobilization
Tissue regeneration
Ethylene Glycol
Phospholipids
Cell growth
poly(lactic acid)
Scaffolds
Immobilization
Lipids
Machinery
Culture Media
Surface treatment

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Cell patterning on polylactic acid through surface-tethered oligonucleotides. / Matsui, Toshiki; Arima, Yusuke; Takemoto, Naohiro; Iwata, Hiroo.

In: Acta Biomaterialia, Vol. 13, 01.02.2015, p. 32-41.

Research output: Contribution to journalArticle

Matsui, Toshiki ; Arima, Yusuke ; Takemoto, Naohiro ; Iwata, Hiroo. / Cell patterning on polylactic acid through surface-tethered oligonucleotides. In: Acta Biomaterialia. 2015 ; Vol. 13. pp. 32-41.
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