Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells

Masao Ichinose, Kazumasa Miki, Masae Tatematsu, Takakazu Mizuno, Mamoru Mutai, Chie Furihata, Yoshikazu Ichihara, Takeshi Ishihara, Masao Tanji, Hiroshi Oka, Yoshikazu Hinohara, Takayuki Takahashi, Takashi Kageyama, Kenji Takahashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.

Original languageEnglish
Pages (from-to)670-677
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number2
DOIs
Publication statusPublished - Sep 15 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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