Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe

Naotaka Tanaka, Atsuro Awai, M. Shah Alam Bhuiyan, Kiyotaka Fujita, Hiroshi Fukui, Kaoru Takegawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have isolated fission yeast mutants that constitutively flocculate upon growth in liquid media. One of these mutants, the gsf1 mutant, was found to cause dominant, nonsexual, and calcium-dependent aggregation of cells into flocs. Its flocculation was inhibited by the addition of galactose but was not affected by the addition of mannose or glucose, unlike Saccharomyces cerevisiae FLO mutants. The gsf1 mutant coflocculated with Schizosaccharomyces pombe wild-type cells, while no coflocculation was found with galactose-deficient (gms1Δ) cells. Moreover, flocculation of the gsf1 mutant was also inhibited by addition of cell wall galactomannan from wild- type cells but not from gms1Δ cells. These results suggested that galactose residues in the cell wall glycoproteins may be receptors of gsf1-mediated flocculation, and therefore cell surface galactosylation is required for nonsexual flocculation in S. pombe.

Original languageEnglish
Pages (from-to)1356-1359
Number of pages4
JournalJournal of bacteriology
Volume181
Issue number4
Publication statusPublished - Feb 1 1999

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Flocculation
Schizosaccharomyces
Galactose
Cell Wall
Cell Aggregation
Mannose
Saccharomyces cerevisiae
Glycoproteins
Calcium
Glucose
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Tanaka, N., Awai, A., Bhuiyan, M. S. A., Fujita, K., Fukui, H., & Takegawa, K. (1999). Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe. Journal of bacteriology, 181(4), 1356-1359.

Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe. / Tanaka, Naotaka; Awai, Atsuro; Bhuiyan, M. Shah Alam; Fujita, Kiyotaka; Fukui, Hiroshi; Takegawa, Kaoru.

In: Journal of bacteriology, Vol. 181, No. 4, 01.02.1999, p. 1356-1359.

Research output: Contribution to journalArticle

Tanaka, N, Awai, A, Bhuiyan, MSA, Fujita, K, Fukui, H & Takegawa, K 1999, 'Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe', Journal of bacteriology, vol. 181, no. 4, pp. 1356-1359.
Tanaka, Naotaka ; Awai, Atsuro ; Bhuiyan, M. Shah Alam ; Fujita, Kiyotaka ; Fukui, Hiroshi ; Takegawa, Kaoru. / Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe. In: Journal of bacteriology. 1999 ; Vol. 181, No. 4. pp. 1356-1359.
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