Cell-to-cell connection of endothelial progenitor cells with cardiac myocytes by nanotubes: A novel mechanism for cell fate changes?

Masamichi Koyanagi, Ralf P. Brandes, Judith Haendeler, Andreas M. Zeiher, Stefanie Dimmeler

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The regeneration of new myocardium by stem or progenitor cells is an important therapeutic option. Cellular or nuclear fusion is considered as an alternative to cell reprogramming by transdifferentiation. However, the generation of hybrid cells may also be a consequence of a transient transmembrane exchange of proteins and organelles between cells. Therefore, we investigated the formation of intercellular connections, which may allow the transport of macromolecular structures between labeled adult human endothelial progenitor cells (EPC) and GFP-expressing neonatal rat cardiomyocytes (CM) in a coculture system. FACS analysis revealed that, 6 days after initiation of coculture, 2.1±0.4% of the cells stained positive for GFP and Dil-ac-LDL. 6 hours after initiation of the coculture, ultrafine intercellular structures between Dil-ac-LDL-labeled EPC and GFP-expressing CM were observed. The number of EPC, which established nanotubular connections with CM increased from 0.5±0.2% after 6 hours to 2.6±03% after 24 hours of coculture. The intercellular connections had a diameter from 50 to 800 nm, a length of 5 to 120 μm, and were only transiently established. To determine whether the nanotubular structures allowed the transport of organelles, we labeled CM with a mitochondrial live tracker (Mito-Tracker). Using time-lapse video microscopy, we observed the transport of stained complexes between CM and EPC resulting in up-take of MitoTracker-positive structures in EPC. Thus, the present study shows a novel type of cell-to-cell communication between progenitor cells and CM in vitro, which may contribute to the acquisition of a cardiomyogenic phenotype independent of permanent cellular or nuclear fusion.

Original languageEnglish
Pages (from-to)1039-1041
Number of pages3
JournalCirculation research
Volume96
Issue number10
DOIs
Publication statusPublished - May 27 2005

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Nanotubes
Cardiac Myocytes
Coculture Techniques
Nuclear Fusion
Stem Cells
Organelles
Cell Transdifferentiation
Video Microscopy
Hybrid Cells
Cell Communication
Endothelial Progenitor Cells
Regeneration
Myocardium
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Cell-to-cell connection of endothelial progenitor cells with cardiac myocytes by nanotubes : A novel mechanism for cell fate changes? / Koyanagi, Masamichi; Brandes, Ralf P.; Haendeler, Judith; Zeiher, Andreas M.; Dimmeler, Stefanie.

In: Circulation research, Vol. 96, No. 10, 27.05.2005, p. 1039-1041.

Research output: Contribution to journalArticle

Koyanagi, Masamichi ; Brandes, Ralf P. ; Haendeler, Judith ; Zeiher, Andreas M. ; Dimmeler, Stefanie. / Cell-to-cell connection of endothelial progenitor cells with cardiac myocytes by nanotubes : A novel mechanism for cell fate changes?. In: Circulation research. 2005 ; Vol. 96, No. 10. pp. 1039-1041.
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