Cell tropism of wild-type measles virus is affected by amino acid substitutions in the P, V and M proteins, or by a truncation in the C protein

Naoko Miyajima, Makoto Takeda, Masato Tashiro, Koji Hashimoto, Yusuke Yanagi, Kyosuke Nagata, Kaoru Takeuchi

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Abstract

Two nucleotide differences in the P/C/V and M genes between B95a cell- and Vero cell-isolated wild-type measles viruses (MV) have previously been found from the same patient. The nucleotide difference in the P/C/V gene resulted in an amino acid difference (M175I) in the P and V proteins and a 19 aa deletion in the C protein. The nucleotide difference in the M gene resulted in an amino acid difference (P64H) in the M protein. To verify this result and to examine further whether the amino acid difference or truncation is important for MV cell tropism, recombinant MV strains containing one of the two nucleotide substitutions, or both, were generated. It was found that the P64H substitution in the M protein was important for efficient virus growth and dissemination in Vero cells and that the M1 75I substitution in the P and V protein or truncation of the C protein was required for optimal growth.

Original languageEnglish
Pages (from-to)3001-3006
Number of pages6
JournalJournal of General Virology
Volume85
Issue number10
DOIs
Publication statusPublished - Oct 1 2004

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All Science Journal Classification (ASJC) codes

  • Virology

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