Cellular balance of glutathione levels through the expression of γ-glutamylcysteine synthetase and glutathione thiol transferase genes in human hepatic cells resistant to a glutathione poison

Toshiya Tanaka, Takeshi Uchiumi, Minoru Nomoto, Kimitoshi Kohno, Takahito Kondo, Kazuto Nishio, Nagahiro Saijo, Michihiko Kuwano

Research output: Contribution to journalArticle

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Abstract

Buthionine sulfoximine (BSO) is a synthetic amino acid that irreversibly inhibits glutathione biosynthesis and deranges reduced glutathione (GSH) metabolism in liver cells. We isolated two BSO-resistant lines, HLE/BSO2-1 and HLE/BSO2-2, from human hepatic HLE/WT cells. Cellular levels of the P(i) class glutathione thiol transferase (GSTP1) were 3-fold lower in BSO-resistant lines than in HLE/WT cells. By contrast, γ-glutamylcysteine synthetase (GCS) heavy subunit (GCSh) mRNA levels were markedly decreased in HLE/BSO2-1 and HLE/BSO2-2 as compared with HLE/WT. The expression of a dominant-negative mutant of c-Jun inhibited the GCSh promoter activity in HLE/WT, but not in HLE/BSO2-1. Cellular levels of AP-1, however, were not decreased in either BSO-resistant cell line. Transfection of GCSh promoter of various lengths driven reporter constructs showed no sequence-specific increase in the promoter activities in HLE/BSO2-1. However, transfection of GSTP1 cDNA into HLE/BSO2-1 and HLE/BSO2-2 restored the levels of GCSh mRNA and the GCSh promoter activity to those of HLE/WT. Sequences between -315 and -241 bp of the 5' region contained an AP-1 site responsible for the enhanced GCSh promoter activity in GSTP1 transfectants of HLE/BSO2-1. In vivo footprint analysis showed a specific protection of the AP-1 site on GCSh promoter in GSTP1 transfected HLE/BSO2-1. GSH homeostasis thus appears to be maintained by an interaction between GSTP1 and GCS in human hepatic cells resistant to the GSH poison. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)367-377
Number of pages11
JournalBiochimica et Biophysica Acta - General Subjects
Volume1427
Issue number3
DOIs
Publication statusPublished - May 24 1999

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Glutamate-Cysteine Ligase
Buthionine Sulfoximine
Poisons
Transferases
Glutathione Transferase
Sulfhydryl Compounds
Glutathione
Hepatocytes
Transcription Factor AP-1
Genes
Transfection
Messenger RNA
Liver
Biosynthesis
Metabolism
Homeostasis
Complementary DNA
Cells
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Cellular balance of glutathione levels through the expression of γ-glutamylcysteine synthetase and glutathione thiol transferase genes in human hepatic cells resistant to a glutathione poison. / Tanaka, Toshiya; Uchiumi, Takeshi; Nomoto, Minoru; Kohno, Kimitoshi; Kondo, Takahito; Nishio, Kazuto; Saijo, Nagahiro; Kuwano, Michihiko.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1427, No. 3, 24.05.1999, p. 367-377.

Research output: Contribution to journalArticle

Tanaka, Toshiya ; Uchiumi, Takeshi ; Nomoto, Minoru ; Kohno, Kimitoshi ; Kondo, Takahito ; Nishio, Kazuto ; Saijo, Nagahiro ; Kuwano, Michihiko. / Cellular balance of glutathione levels through the expression of γ-glutamylcysteine synthetase and glutathione thiol transferase genes in human hepatic cells resistant to a glutathione poison. In: Biochimica et Biophysica Acta - General Subjects. 1999 ; Vol. 1427, No. 3. pp. 367-377.
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AU - Tanaka, Toshiya

AU - Uchiumi, Takeshi

AU - Nomoto, Minoru

AU - Kohno, Kimitoshi

AU - Kondo, Takahito

AU - Nishio, Kazuto

AU - Saijo, Nagahiro

AU - Kuwano, Michihiko

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AB - Buthionine sulfoximine (BSO) is a synthetic amino acid that irreversibly inhibits glutathione biosynthesis and deranges reduced glutathione (GSH) metabolism in liver cells. We isolated two BSO-resistant lines, HLE/BSO2-1 and HLE/BSO2-2, from human hepatic HLE/WT cells. Cellular levels of the P(i) class glutathione thiol transferase (GSTP1) were 3-fold lower in BSO-resistant lines than in HLE/WT cells. By contrast, γ-glutamylcysteine synthetase (GCS) heavy subunit (GCSh) mRNA levels were markedly decreased in HLE/BSO2-1 and HLE/BSO2-2 as compared with HLE/WT. The expression of a dominant-negative mutant of c-Jun inhibited the GCSh promoter activity in HLE/WT, but not in HLE/BSO2-1. Cellular levels of AP-1, however, were not decreased in either BSO-resistant cell line. Transfection of GCSh promoter of various lengths driven reporter constructs showed no sequence-specific increase in the promoter activities in HLE/BSO2-1. However, transfection of GSTP1 cDNA into HLE/BSO2-1 and HLE/BSO2-2 restored the levels of GCSh mRNA and the GCSh promoter activity to those of HLE/WT. Sequences between -315 and -241 bp of the 5' region contained an AP-1 site responsible for the enhanced GCSh promoter activity in GSTP1 transfectants of HLE/BSO2-1. In vivo footprint analysis showed a specific protection of the AP-1 site on GCSh promoter in GSTP1 transfected HLE/BSO2-1. GSH homeostasis thus appears to be maintained by an interaction between GSTP1 and GCS in human hepatic cells resistant to the GSH poison. Copyright (C) 1999 Elsevier Science B.V.

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