Cellular events in the normal and inflamed cornea

Kohei Sonoda, shintaro nakao, Takahiro Nakamura, Toru Oshima, Hong Qiao, Toshio Hisatomi, Shigeru Kinoshita, Tatsuro Ishibashi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Purpose: Although normal cornea is an avascular transparent structure, bone marrow (BM)-derived cells exist in situ even in noninflamed conditions. We evaluated constitutive cellular trafficking into the cornea in the naive state and investigated how corneal inflammation may be initiated by various stimuli. Methods: BM chimeric mice were generated using BM from enhanced green fluorescent protein (eGFP) transgenic mice. Corneas of chimeric mice were carefully studied by fluorescent biomicroscopy until 6 months after transplantation. To analyze initiation of cellular events in corneal immune response, we cauterized the center of the cornea or inoculated IL-1β into the corneal micropocket. Cellular events were analyzed by flow cytometry. Results: At 2 weeks after BM transplantation, GFP+ cells gradually migrated into the cornea from the limbal area and were distributed over the entire cornea at 6 months. In both the cauterization and micropocket assays, infiltration of neutrophils and macrophages occurred on days 2 and 4, respectively. Depletion of neutrophils by anti-Gr-1Ab significantly reduced corneal edema/opacity induced by cauterization. IL-1β-induced angiogenesis was markedly reduced in monocyte chemoattractant protein-1 knockout mice. Conclusions: BM-derived cells are recruited into the normal cornea and may be essential to maintain corneal clarity. In the inflamed cornea, neutrophils might be responsible for acute corneal edema/opacity and macrophages for corneal angiogenesis and chronic inflammation.

Original languageEnglish
JournalCornea
Volume24
Issue number8 SUPPL.
Publication statusPublished - Nov 1 2005

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Cornea
Corneal Edema
Corneal Opacity
Cautery
Interleukin-1
Bone Marrow Cells
Neutrophils
Bone Marrow
Macrophages
Corneal Neovascularization
Inflammation
Neutrophil Infiltration
Chemokine CCL2
Bone Marrow Transplantation
Knockout Mice
Transgenic Mice
Flow Cytometry
Transplantation

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Sonoda, K., nakao, S., Nakamura, T., Oshima, T., Qiao, H., Hisatomi, T., ... Ishibashi, T. (2005). Cellular events in the normal and inflamed cornea. Cornea, 24(8 SUPPL.).

Cellular events in the normal and inflamed cornea. / Sonoda, Kohei; nakao, shintaro; Nakamura, Takahiro; Oshima, Toru; Qiao, Hong; Hisatomi, Toshio; Kinoshita, Shigeru; Ishibashi, Tatsuro.

In: Cornea, Vol. 24, No. 8 SUPPL., 01.11.2005.

Research output: Contribution to journalArticle

Sonoda, K, nakao, S, Nakamura, T, Oshima, T, Qiao, H, Hisatomi, T, Kinoshita, S & Ishibashi, T 2005, 'Cellular events in the normal and inflamed cornea', Cornea, vol. 24, no. 8 SUPPL..
Sonoda K, nakao S, Nakamura T, Oshima T, Qiao H, Hisatomi T et al. Cellular events in the normal and inflamed cornea. Cornea. 2005 Nov 1;24(8 SUPPL.).
Sonoda, Kohei ; nakao, shintaro ; Nakamura, Takahiro ; Oshima, Toru ; Qiao, Hong ; Hisatomi, Toshio ; Kinoshita, Shigeru ; Ishibashi, Tatsuro. / Cellular events in the normal and inflamed cornea. In: Cornea. 2005 ; Vol. 24, No. 8 SUPPL.
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AU - Hisatomi, Toshio

AU - Kinoshita, Shigeru

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