Central role of vascular smooth muscle hyperreactivity in coronary hyperconstriction after balloon injury in miniature pigs

Takeshi Kuga, Toshiaki Kadokami, Kouichi Kuwata, Hiroshi Hata, Yuichi Ohara, Kensuke Egashira, Hiroaki Shimokawa, Akira Takeshita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. Coronary constrictive responses to autacoids become augmented 1 week after balloon injury in our swine model. The present study aimed to elucidate the mechanisms of this effect. Methods. In 12 hypercholesterolaemic miniature pigs, the coronary constrictive response to serotonin was examined angiographically 1 week after injury. After the angiographic study, organ chamber experiments using excised coronary artery were performed to clarify whether functional changes in endothelial cells or in vascular smooth muscle cells contributed to the hyperconstriction. Results. The coronary constrictive response to serotonin in vivo was significantly greater at the previously injured site than at the non-injured site. The degree of the hyperconstriction at the previously injured site exceeded that predicted from a geometric theory. Histological examination demonstrated that the previously injured site was almost covered with regenerated endothelial cells. In vitro studies demonstrated that serotonin caused significantly greater contraction in coronary artery strips, whether with or without endothelium, from the previously injured site than in those from the non-injured site. Endothelium-dependent relaxation in response to serotonin was comparable at the injured and non-injured sites. Conclusions. These results suggest that the coronary hyperconstriction response to serotonin 1 week after injury results primarily from hyperreactivity of vascular smooth muscle. Whereas any contribution of endothelial dysfunction or the geometric effect may be minimal.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalCoronary Artery Disease
Volume8
Issue number2
Publication statusPublished - Jun 25 1997

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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    Kuga, T., Kadokami, T., Kuwata, K., Hata, H., Ohara, Y., Egashira, K., Shimokawa, H., & Takeshita, A. (1997). Central role of vascular smooth muscle hyperreactivity in coronary hyperconstriction after balloon injury in miniature pigs. Coronary Artery Disease, 8(2), 69-75.