Centrosome abnormalities in pancreatic ductal carcinoma

Norihiro Sato, Kazuhiro Mizumoto, Masafumi Nakamura, Kenjiro Nakamura, Masahiro Kusumoto, Hideaki Niiyama, Takahiro Ogawa, Masao Tanaka

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101 Citations (Scopus)

Abstract

The centrosome plays an important role in microtubule nucleation and organization, ensuring the establishment of cell polarity and balanced chromosome segregation. Recent studies have suggested that the loss of cell polarity and/or chromosome missegregation (aneuploidy) in human malignant tumors could result from defects in centrosome function. Using immunofluorescence analysis with an antibody to γ-tubulin (a well- characterized centrosomal component), we examined surgically resected human pancreatic tissues for centrosome abnormalities. The tissues included ductal carcinomas (n = 13), adenomas (n = 3), endocrine tumors (n = 3), chronic pancreatitis (n = 5), and normal pancreatic tissues (n = 12). We found that most (85%) carcinomas and some adenomas displayed abnormal centrosome profiles, characterized by an increase in size and number of centrosomes, and by their irregular distribution. In contrast, none of normal ductal and stromal tissues showed these abnormalities. These findings suggest that centrosome abnormalities may develop at a relatively early stage of pancreatic ductal carcinogenesis.

Original languageEnglish
Pages (from-to)963-970
Number of pages8
JournalClinical Cancer Research
Volume5
Issue number5
Publication statusPublished - May 1 1999

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Sato, N., Mizumoto, K., Nakamura, M., Nakamura, K., Kusumoto, M., Niiyama, H., Ogawa, T., & Tanaka, M. (1999). Centrosome abnormalities in pancreatic ductal carcinoma. Clinical Cancer Research, 5(5), 963-970.