Centrosome amplification as a putative prognostic biomarker for the classification of urothelial carcinomas

Yoshiaki Yamamoto, Taku Misumi, Satoshi Eguchi, Yasuyo Chochi, Seiji Kitahara, Motonao Nakao, Kazuhiro Nagao, Takahiko Hara, Shigeru Sakano, Tomoko Furuya, Atsunori Oga, Shigeto Kawauchi, Kohsuke Sasaki, Hideyasu Matsuyama

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Recent studies have reported that centrosome amplification is closely related to chromosomal instability and patient prognosis in human malignancies. The purpose of this study was to elucidate the relationship between centrosome amplification and genomic alterations in urothelial carcinomas. Centrosomes were evaluated by immunohistochemistry using anti-γ-tubulin antibody. Array-based comparative genomic hybridization technology using DNA chips spotted with 4030 bacterial artificial chromosome clones was applied to 70 urothelial carcinomas to examine DNA copy number aberrations. Studying aberrations in the number of chromosomes 7, 9, and 17 using fluorescence in situ hybridization allowed the estimation of the degree of chromosomal instability. DNA copy number gains at 20p12.2, 5p15.2, 5p15.31, and 17q25.3 and losses at 17p12, 8p22, 2q37.3, 5q31.1, and 2q37.3 were more frequent in tumors with centrosome amplification than in those without it. The total numbers of DNA copy number aberrations and frequency of chromosomal instability were also larger in tumors with centrosome amplification than in those without it (P =.0263 and P <.0001, respectively). These parameters were more closely associated with centrosome amplification than with the subjectively assigned tumor grade (P =.0405 and P =.0020, respectively). Thus, these data suggest that centrosome amplification may have great potential as a biomarker for improved objective classification of urothelial carcinoma and estimation of prognosis.

Original languageEnglish
Pages (from-to)1923-1930
Number of pages8
JournalHuman Pathology
Volume42
Issue number12
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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