Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa

Nozomu Okino, Makoto Ito

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We previously reported the purification, molecular cloning, and characterization of a neutral ceramidase from Pseudomonas aeruginosa strain AN17 (Okino, N., Tani, M., Imayama, S., and Ito, M. (1998) J. Biol. Chem. 273, 14368-14373; Okino, N., Ichinose, S., Omori, A., Imayama, S., Nakamura, T., and Ito, M. (1999) J. Biol. Chem. 274, 36616-36622). Interestingly, the gene encoding the enzyme is adjacent to that encoding hemolytic phospholipase C (plcH) in the genome of Pseudomonas aeruginosa, which is a well known pathogen for opportunistic infections. Wereport here that simultaneous production of PlcH and ceramidase was induced by several lipids and PlcH-induced hemolysis was significantly enhanced by the action of the ceramidase. When the strain was cultured with sphingomyelin or phosphatidylcholine, production of both enzymes drastically increased, causing the increase of hemolytic activity in the cellfree culture supernatant. Ceramide and sphingosine were also effective in promoting the production of ceramidase but not that of PlcH. Furthermore, we found that the hemolytic activity of a Bacillus cereus sphingomyelinase was significantly enhanced by addition of a recombinant Pseudomonas ceramidase. TLC analysis of the erythrocytes showed that ceramide produced from sphingomyelin by the sphingomyelinase was partly converted to sphingosine by the ceramidase. A ceramidase-null mutant strain caused much less hemolysis of sheep erythrocytes than did the wild-type strain. Sphingosine was detected in the erythrocytes co-cultured with the wild-type strain but not the mutant strain. Finally, we found that the enhancement of PlcH-induced hemolysis by the ceramidase occurred in not only sheep but also human erythrocytes. These results may indicate that the ceramidase enhances the PlcH-induced cytotoxicity and provide newinsightsintotheroleofsphingolipid-degradingenzymesinthe pathogenicity of P. aeruginosa.

Original languageEnglish
Pages (from-to)6021-6030
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number9
DOIs
Publication statusPublished - Mar 2 2007

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Ceramidases
Type C Phospholipases
Hemolysis
Pseudomonas aeruginosa
Sphingosine
Erythrocytes
Sphingomyelin Phosphodiesterase
Sphingomyelins
Ceramides
Neutral Ceramidase
Sheep
Bacillus cereus
Gene encoding
Cloning
Opportunistic Infections
Molecular Cloning
Pathogens
Enzymes
Cytotoxicity
Pseudomonas

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa. / Okino, Nozomu; Ito, Makoto.

In: Journal of Biological Chemistry, Vol. 282, No. 9, 02.03.2007, p. 6021-6030.

Research output: Contribution to journalArticle

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