Cerebral ischemia combined with β-amyloid impairs spatial memory in the eight-arm radial maze task in rats

Katsunori Iwasaki, Nobuaki Egashira, Izzettin Hatip-Al-Khatib, Yuki Akiyoshi, Takashi Arai, Yuki Takagaki, Takuya Watanabe, Kenichi Mishima, Michihiro Fujiwara

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

β-Amyloid (Aβ), a major component of senile plaques in Alzheimer's disease, has been implicated in neuronal cell death, a characteristic feature of this condition. In our previous experiments using primary cultures of hippocampal neurons, Aβ treatment induced neuronal cell death, displaying morphological characteristics of apoptosis that was significantly enhanced by hypoxia. Based on these results, we developed a simple in vivo rat model of Alzheimer's disease using cerebral ischemia, instead of hypoxia, combined with continuous intracerebroventricular administration of Aβ. The combination of cerebral ischemia and Aβ administration, but not either treatment alone, significantly impaired spatial memory in an eight-arm radial maze. A microdialysis study showed that spontaneous release of acetylcholine (ACh) from the dorsal hippocampus had a tendency to decrease in response to Aβ treatment alone or the combination of ischemia and Aβ. High K+-evoked increase in ACh release had a tendency to be inhibited by either ischemia or Aβ treatment alone and was significantly inhibited by the combination of both. Moreover, combination of ischemia and Aβ induced apoptosis of pyramidal neurons in the CA1 region of the hippocampus. Donepezil, a drug currently in clinical use for Alzheimer's disease, improved the impairment of spatial memory induced by cerebral ischemia combined with Aβ. These findings suggest that ischemia is an important factor facilitating the symptoms of Alzheimer's disease, and this model may be useful for developing new drugs for the treatment of Alzheimer's disease.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalBrain Research
Volume1097
Issue number1
DOIs
Publication statusPublished - Jun 30 2006
Externally publishedYes

Fingerprint

Brain Ischemia
Amyloid
Alzheimer Disease
Ischemia
Acetylcholine
Hippocampus
Cell Death
Apoptosis
Brain Hypoxia-Ischemia
Pyramidal Cells
Amyloid Plaques
Microdialysis
Pharmaceutical Preparations
Spatial Memory
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Cerebral ischemia combined with β-amyloid impairs spatial memory in the eight-arm radial maze task in rats. / Iwasaki, Katsunori; Egashira, Nobuaki; Hatip-Al-Khatib, Izzettin; Akiyoshi, Yuki; Arai, Takashi; Takagaki, Yuki; Watanabe, Takuya; Mishima, Kenichi; Fujiwara, Michihiro.

In: Brain Research, Vol. 1097, No. 1, 30.06.2006, p. 216-223.

Research output: Contribution to journalArticle

Iwasaki, K, Egashira, N, Hatip-Al-Khatib, I, Akiyoshi, Y, Arai, T, Takagaki, Y, Watanabe, T, Mishima, K & Fujiwara, M 2006, 'Cerebral ischemia combined with β-amyloid impairs spatial memory in the eight-arm radial maze task in rats', Brain Research, vol. 1097, no. 1, pp. 216-223. https://doi.org/10.1016/j.brainres.2006.04.073
Iwasaki, Katsunori ; Egashira, Nobuaki ; Hatip-Al-Khatib, Izzettin ; Akiyoshi, Yuki ; Arai, Takashi ; Takagaki, Yuki ; Watanabe, Takuya ; Mishima, Kenichi ; Fujiwara, Michihiro. / Cerebral ischemia combined with β-amyloid impairs spatial memory in the eight-arm radial maze task in rats. In: Brain Research. 2006 ; Vol. 1097, No. 1. pp. 216-223.
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