Cerebroside Langmuir monolayers originated from the echinoderms: I. Binary systems of cerebrosides and phospholipids

Hiromichi Nakahara, Shohei Nakamura, Kazufumi Nakamura, Masanori Inagaki, Mariko Aso, Ryuichi Higuchi, Osamu Shibata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The surface pressure (π)-area (A), the surface potential (ΔV)-A and the dipole moment (μ)-A isotherms were obtained for two-component monolayers of two different cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (ΔV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort [J. Colloid Interf. Sci. 46 (1974) 191-202]. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (μ ) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (Xphospholipid), using the additivity rule. From the A-Xphospholipid and ΔVm-X phospholipid plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (ξ) and the interaction energy (-Δε) between the cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.

Original languageEnglish
Pages (from-to)157-174
Number of pages18
JournalColloids and Surfaces B: Biointerfaces
Volume42
Issue number2
DOIs
Publication statusPublished - May 10 2005

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Cerebroside Langmuir monolayers originated from the echinoderms: I. Binary systems of cerebrosides and phospholipids'. Together they form a unique fingerprint.

  • Cite this