Change in Catalytic Property of Trypsin Immobilized on Support Activated with Glutaraldehyde in Lower Protogenic Solvent

Tomoyuki Oki, Kiyoshi Matsumoto, Toshiro Matsui, Yutaka Osajima

Research output: Contribution to journalArticlepeer-review

Abstract

A variety of glutaraldehyde (GA) activated-aminopropyl controlled pore glass supports were prepared in lower protogenic solvents (methanol, ethanol, and n-propanol). When trypsin was used as the ligand, the amount of trypsin immobilized on them decreased in the order of water, methanol, ethanol, and n-propanol; the maximal reduction of 22% was observed in n-propanol versus that in water. The Michaelis constants of the trypsins immobilized on the support activated with GA in alcohols, especially ethanol and propanol, were smaller than that in water, and the minimum Km value of 1.84X10-1 mM was obtained in propanol (4.29X10-1 mM in water). During the reaction of GA with 2-aminoethanol, the amount of the aldehyde group from GA being capable of reacting with 2-aminoethanol significantly decreased when the reaction was done in n-propanol (0.18 mmol/assay volume, cf. in water; 035 mmol/assay volume). The gel permeation chromatography (GPC) measurement of the GA complex, glycine-GA-phenetylamine, also revealed the suppression of the GA self-polymerization in the lower protogenic alcohol as a support activating solvent.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalFood Science and Technology Research
Volume5
Issue number1
DOIs
Publication statusPublished - Feb 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Fingerprint Dive into the research topics of 'Change in Catalytic Property of Trypsin Immobilized on Support Activated with Glutaraldehyde in Lower Protogenic Solvent'. Together they form a unique fingerprint.

Cite this