Change in phenoloxidase and its precursor during silkworm (a80 strain) development

Kohji Yamamoto, Hiroshi Fujii, Takahiro Kusakabe, Katsumi Koga, Yoichi Aso, Masatsune Ishiguro

Research output: Contribution to journalArticle

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Abstract

The changes in phenoloxidase and its precursor, so-called prophenoloxidase, of the silkworm, Bombyx mori (a80 strain) were examined using detection assay with or without detergent to distinguish PO and pro-PO in hemolymph (Yamamoto, et al., 1999). Little or no phenoloxidase activity was detected in hemolymph at the silkworm development during the final larval instar and pupal stage. On the other hand, prophenoloxidase was detected for same time frame. In males, after prophenoloxidase increased slightly at day 4, it decreased to a minimum level at spinning and increased to a maximum level on the day of pupation and decreased gradually to day 5 after pupation. In females, prophenoloxidase decreased gradually to a minimum level on day of spinning and a slight increase was observed at day 8 and it increased to maximum level between day 2 and 3 after pupation and decreased thereafter. Prophenoloxidase mRNA expression was preceded a day or two before, when comparing the change of phenoloxidase mRNA with its product.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number2
Publication statusPublished - Feb 1 2001

Fingerprint

prophenoloxidase
Bombyx
Monophenol Monooxygenase
monophenol monooxygenase
silkworms
pupation
Hemolymph
spinning
hemolymph
Messenger RNA
Bombyx mori
detergents
Detergents
instars
pro-phenoloxidase
assays

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Change in phenoloxidase and its precursor during silkworm (a80 strain) development. / Yamamoto, Kohji; Fujii, Hiroshi; Kusakabe, Takahiro; Koga, Katsumi; Aso, Yoichi; Ishiguro, Masatsune.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 45, No. 2, 01.02.2001, p. 487-493.

Research output: Contribution to journalArticle

Yamamoto, Kohji ; Fujii, Hiroshi ; Kusakabe, Takahiro ; Koga, Katsumi ; Aso, Yoichi ; Ishiguro, Masatsune. / Change in phenoloxidase and its precursor during silkworm (a80 strain) development. In: Journal of the Faculty of Agriculture, Kyushu University. 2001 ; Vol. 45, No. 2. pp. 487-493.
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