Change in the telomere length distribution with age in the Japanese population

Jing Zhi Guan, Toyoki Maeda, Masahiro Sugano, Jun Ichi Oyama, Yoshihiro Higuchi, Naoki Makino

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Telomeres play a role in cellular aging and they may also contribute to the genetic basis of human aging and longevity. A gradual loss of the telomeric repeat sequences has been reported in adult tissue specimens. This study determined the percentage of telomere restriction fragment in various molecular-sized regions in addition to measuring the average telomere length. Mean telomere restriction fragment (TRF) length was determined by Southern blot analysis using a longer telomeric repeat probe with higher sensitivity. A significant decrease in longer telomere fragments and a quick increase in the shortest fragments were observed, especially in male subjects. There was a tendency that the age-adjusted telomere length was longer in females than that observed in males, while males lose the telomeric sequence faster than females. These data indicated that the percentage of longer telomeres fragments decreased, while the shortest fragments increased quickly with age. In addition, the longest telomere fragments decreased and the short fragments increased with a relatively stable frequency with age. There was also a significant difference in the longest telomere fragment percentage between males and female in their 40s and 50s, whereas no difference was observed in the mean TRF length. Interestingly, the changing rate of the longest and the shortest range group of TRF percentage associated with aging seemed quite different between before and after 50-year old with a gender-related contrast. This contrast implies a drastic change around the age of 50 of unknown factors that affect telomere attrition.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalMolecular and cellular biochemistry
Volume304
Issue number1-2
DOIs
Publication statusPublished - Oct 1 2007

Fingerprint

Age Distribution
Telomere
Aging of materials
Population
Tissue
Cell Aging
Medical Genetics
Southern Blotting

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Change in the telomere length distribution with age in the Japanese population. / Guan, Jing Zhi; Maeda, Toyoki; Sugano, Masahiro; Oyama, Jun Ichi; Higuchi, Yoshihiro; Makino, Naoki.

In: Molecular and cellular biochemistry, Vol. 304, No. 1-2, 01.10.2007, p. 353-360.

Research output: Contribution to journalArticle

Guan, Jing Zhi ; Maeda, Toyoki ; Sugano, Masahiro ; Oyama, Jun Ichi ; Higuchi, Yoshihiro ; Makino, Naoki. / Change in the telomere length distribution with age in the Japanese population. In: Molecular and cellular biochemistry. 2007 ; Vol. 304, No. 1-2. pp. 353-360.
@article{64b7d885dbc84e7abb8af65ed2320fe9,
title = "Change in the telomere length distribution with age in the Japanese population",
abstract = "Telomeres play a role in cellular aging and they may also contribute to the genetic basis of human aging and longevity. A gradual loss of the telomeric repeat sequences has been reported in adult tissue specimens. This study determined the percentage of telomere restriction fragment in various molecular-sized regions in addition to measuring the average telomere length. Mean telomere restriction fragment (TRF) length was determined by Southern blot analysis using a longer telomeric repeat probe with higher sensitivity. A significant decrease in longer telomere fragments and a quick increase in the shortest fragments were observed, especially in male subjects. There was a tendency that the age-adjusted telomere length was longer in females than that observed in males, while males lose the telomeric sequence faster than females. These data indicated that the percentage of longer telomeres fragments decreased, while the shortest fragments increased quickly with age. In addition, the longest telomere fragments decreased and the short fragments increased with a relatively stable frequency with age. There was also a significant difference in the longest telomere fragment percentage between males and female in their 40s and 50s, whereas no difference was observed in the mean TRF length. Interestingly, the changing rate of the longest and the shortest range group of TRF percentage associated with aging seemed quite different between before and after 50-year old with a gender-related contrast. This contrast implies a drastic change around the age of 50 of unknown factors that affect telomere attrition.",
author = "Guan, {Jing Zhi} and Toyoki Maeda and Masahiro Sugano and Oyama, {Jun Ichi} and Yoshihiro Higuchi and Naoki Makino",
year = "2007",
month = "10",
day = "1",
doi = "10.1007/s11010-007-9518-2",
language = "English",
volume = "304",
pages = "353--360",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Change in the telomere length distribution with age in the Japanese population

AU - Guan, Jing Zhi

AU - Maeda, Toyoki

AU - Sugano, Masahiro

AU - Oyama, Jun Ichi

AU - Higuchi, Yoshihiro

AU - Makino, Naoki

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Telomeres play a role in cellular aging and they may also contribute to the genetic basis of human aging and longevity. A gradual loss of the telomeric repeat sequences has been reported in adult tissue specimens. This study determined the percentage of telomere restriction fragment in various molecular-sized regions in addition to measuring the average telomere length. Mean telomere restriction fragment (TRF) length was determined by Southern blot analysis using a longer telomeric repeat probe with higher sensitivity. A significant decrease in longer telomere fragments and a quick increase in the shortest fragments were observed, especially in male subjects. There was a tendency that the age-adjusted telomere length was longer in females than that observed in males, while males lose the telomeric sequence faster than females. These data indicated that the percentage of longer telomeres fragments decreased, while the shortest fragments increased quickly with age. In addition, the longest telomere fragments decreased and the short fragments increased with a relatively stable frequency with age. There was also a significant difference in the longest telomere fragment percentage between males and female in their 40s and 50s, whereas no difference was observed in the mean TRF length. Interestingly, the changing rate of the longest and the shortest range group of TRF percentage associated with aging seemed quite different between before and after 50-year old with a gender-related contrast. This contrast implies a drastic change around the age of 50 of unknown factors that affect telomere attrition.

AB - Telomeres play a role in cellular aging and they may also contribute to the genetic basis of human aging and longevity. A gradual loss of the telomeric repeat sequences has been reported in adult tissue specimens. This study determined the percentage of telomere restriction fragment in various molecular-sized regions in addition to measuring the average telomere length. Mean telomere restriction fragment (TRF) length was determined by Southern blot analysis using a longer telomeric repeat probe with higher sensitivity. A significant decrease in longer telomere fragments and a quick increase in the shortest fragments were observed, especially in male subjects. There was a tendency that the age-adjusted telomere length was longer in females than that observed in males, while males lose the telomeric sequence faster than females. These data indicated that the percentage of longer telomeres fragments decreased, while the shortest fragments increased quickly with age. In addition, the longest telomere fragments decreased and the short fragments increased with a relatively stable frequency with age. There was also a significant difference in the longest telomere fragment percentage between males and female in their 40s and 50s, whereas no difference was observed in the mean TRF length. Interestingly, the changing rate of the longest and the shortest range group of TRF percentage associated with aging seemed quite different between before and after 50-year old with a gender-related contrast. This contrast implies a drastic change around the age of 50 of unknown factors that affect telomere attrition.

UR - http://www.scopus.com/inward/record.url?scp=34648843368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34648843368&partnerID=8YFLogxK

U2 - 10.1007/s11010-007-9518-2

DO - 10.1007/s11010-007-9518-2

M3 - Article

C2 - 17594061

AN - SCOPUS:34648843368

VL - 304

SP - 353

EP - 360

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -