Changes in blood pancreatic polypeptide and ghrelin concentrations in response to feeding in sheep

Hideyuki Takahashi, Y. Kurose, Y. Suzuki, M. Kojima, T. Yamaguchi, Y. Yoshida, Y. Azuma, T. Sugino, M. Kojima, K. Kangawa, Y. Hasegawa, S. Kobayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The roles of pancreatic polypeptide (PP) have not been determined in ruminant animals. The aim of the present study was to examine the role of PP in the regulation of ghrelin secretion in sheep. Two experiments were conducted using four 2-yr-old Suffolk wethers fed a maintenance diet of alfalfa hay cubes. In Exp. 1, the effects of feeding on blood ghrelin and PP concentrations were examined in scheduled-fed sheep. Blood samples were collected every 10 min from 30 min before to 360 min after feeding. Plasma PP concentra-tions were transiently increased from the preprandial average value to the values from 30 to 60 min after feeding and gradually decreased (P < 0.05) to stable values from 150 to 180 min. The values from 30 to 60 min were greater (P < 0.05) than those from 150 to 360 min. In contrast, plasma ghrelin concentrations were gradually decreased (P < 0.01) by feeding. The values from 60 to 360 min were less (P < 0.01) than the preprandial average value. In Exp. 2, the effects of con-tinuous PP infusion on ghrelin secretion were examined in feed-deprived sheep. The animals were deprived of feed for 48 h before PP infusion. The PP-treated group intravenously received synthetic bovine PP at a rate of 10 pmol·kg -1 of BW·min -1 for 180 min. Blood samples were collected every 10 min from 30 min before to 180 min after the commencement of PP infusion. Plasma PP concentrations reached a plateau within 30 min af-ter the commencement of PP infusion. Plasma ghrelin concentrations were decreased (P = 0.002, 0.016, 0.007) by PP infusion at 160, 170, and 180 min, respectively. In conclusion, plasma ghrelin and PP concentrations were decreased and increased, respectively, in response to feeding in ruminant animals. Furthermore, PP could depress ghrelin secretion.

Original languageEnglish
Pages (from-to)2103-2107
Number of pages5
JournalJournal of animal science
Volume88
Issue number6
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

Fingerprint

sheep feeding
pancreatic polypeptide
Pancreatic Polypeptide
ghrelin
Ghrelin
Sheep
blood
Ruminants
secretion
sheep
Suffolk (sheep breed)
animals
Medicago sativa
animal feeding
alfalfa hay

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Changes in blood pancreatic polypeptide and ghrelin concentrations in response to feeding in sheep. / Takahashi, Hideyuki; Kurose, Y.; Suzuki, Y.; Kojima, M.; Yamaguchi, T.; Yoshida, Y.; Azuma, Y.; Sugino, T.; Kojima, M.; Kangawa, K.; Hasegawa, Y.; Kobayashi, S.

In: Journal of animal science, Vol. 88, No. 6, 01.06.2010, p. 2103-2107.

Research output: Contribution to journalArticle

Takahashi, H, Kurose, Y, Suzuki, Y, Kojima, M, Yamaguchi, T, Yoshida, Y, Azuma, Y, Sugino, T, Kojima, M, Kangawa, K, Hasegawa, Y & Kobayashi, S 2010, 'Changes in blood pancreatic polypeptide and ghrelin concentrations in response to feeding in sheep', Journal of animal science, vol. 88, no. 6, pp. 2103-2107. https://doi.org/10.2527/jas.2009-1920
Takahashi, Hideyuki ; Kurose, Y. ; Suzuki, Y. ; Kojima, M. ; Yamaguchi, T. ; Yoshida, Y. ; Azuma, Y. ; Sugino, T. ; Kojima, M. ; Kangawa, K. ; Hasegawa, Y. ; Kobayashi, S. / Changes in blood pancreatic polypeptide and ghrelin concentrations in response to feeding in sheep. In: Journal of animal science. 2010 ; Vol. 88, No. 6. pp. 2103-2107.
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abstract = "The roles of pancreatic polypeptide (PP) have not been determined in ruminant animals. The aim of the present study was to examine the role of PP in the regulation of ghrelin secretion in sheep. Two experiments were conducted using four 2-yr-old Suffolk wethers fed a maintenance diet of alfalfa hay cubes. In Exp. 1, the effects of feeding on blood ghrelin and PP concentrations were examined in scheduled-fed sheep. Blood samples were collected every 10 min from 30 min before to 360 min after feeding. Plasma PP concentra-tions were transiently increased from the preprandial average value to the values from 30 to 60 min after feeding and gradually decreased (P < 0.05) to stable values from 150 to 180 min. The values from 30 to 60 min were greater (P < 0.05) than those from 150 to 360 min. In contrast, plasma ghrelin concentrations were gradually decreased (P < 0.01) by feeding. The values from 60 to 360 min were less (P < 0.01) than the preprandial average value. In Exp. 2, the effects of con-tinuous PP infusion on ghrelin secretion were examined in feed-deprived sheep. The animals were deprived of feed for 48 h before PP infusion. The PP-treated group intravenously received synthetic bovine PP at a rate of 10 pmol·kg -1 of BW·min -1 for 180 min. Blood samples were collected every 10 min from 30 min before to 180 min after the commencement of PP infusion. Plasma PP concentrations reached a plateau within 30 min af-ter the commencement of PP infusion. Plasma ghrelin concentrations were decreased (P = 0.002, 0.016, 0.007) by PP infusion at 160, 170, and 180 min, respectively. In conclusion, plasma ghrelin and PP concentrations were decreased and increased, respectively, in response to feeding in ruminant animals. Furthermore, PP could depress ghrelin secretion.",
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AU - Yamaguchi, T.

AU - Yoshida, Y.

AU - Azuma, Y.

AU - Sugino, T.

AU - Kojima, M.

AU - Kangawa, K.

AU - Hasegawa, Y.

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