Changes in element concentrations induced by agonist in pig pancreatic acinar cells

Sadao Sasaki, Ikuko Nakagaki, Hisao Kondo, Seiki Hori

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Changes in electrolytes of pig pancreatic acinar cells following application of gastrin-cholecystokinin (CCK) were investigated using the technique of X-ray microanalysis of hydrated and dehydrated sections of freshly frozen pancreas. After stimulation by CCK (10-9 M), Na and Cl increased significantly in the cytoplasm [Na, from 10 mmol/kg wet wt. (48 mmol/kg dry wt.) to 19 mmol/kg (95 mmol/kg); Cl, from 22 mmol/kg (105 mmol/kg) to 49 mmol/kg (245 mmol/kg)] as well as in the luminal interspace [Na, from 53 mmol/kg (189 mmol/kg) to 65 mmol/kg (283 mmol/kg); Cl, from 65 mmol/kg (232 mmol/kg) to 102 mmol/kg (443 mmol/kg)]. In the secretory granules Cl increased significantly from 30 mmol/kg (86 mmol/kg) to 67 mmol/kg (203 mmol/kg). K decreased significantly from 120 mmol/kg (571 mmol/kg) to 81 mmol/kg (405 mmol/kg) in the cytoplasm, while both increased from 38 mmol/kg (109 mmol/kg) to 58 mmol/kg (176 mmol/kg) in the granules and from 46 mmol/kg (164 mmol/kg) to 48 mmol/kg (209 mmol/kg) in the luminal interspace. Ca increased significantly in the cytoplasm as well as in the luminal interspace, and decreased significantly in the secretory granules. CCK evoked Ca release from secretory granules in the secretory pole of acinar cells. The values were measured from dehydrated sections, and agreed well with those from hydrated sections. The effect of furosemide, an inhibitor of the Na+-K+-2Cl- co-transporter, on the ion transport of acinar cell was studied. When furosemide (10-5 M) was added to the external solution, the cytoplasmic Cl and Ca concentrations decreased significantly, while there was a little decrease in Na and K concentrations under the secretory condition. These results indicate that Na+-K+-2Cl- co-transport, and Na+, Cl- and K+ exits into the lumen are involved in the mechanism of ion secretion in pig pancreatic acinar cells.

Original languageEnglish
Pages (from-to)538-545
Number of pages8
JournalPflugers Archiv European Journal of Physiology
Volume432
Issue number3
DOIs
Publication statusPublished - Aug 7 1996
Externally publishedYes

Fingerprint

Acinar Cells
Cholecystokinin
Secretory Vesicles
Swine
Furosemide
Cytoplasm
Ions
Symporters
Gastrins
Electron Probe Microanalysis
Electrolytes
Poles
Ion Transport
Frozen Sections
Pancreas
X rays

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Changes in element concentrations induced by agonist in pig pancreatic acinar cells. / Sasaki, Sadao; Nakagaki, Ikuko; Kondo, Hisao; Hori, Seiki.

In: Pflugers Archiv European Journal of Physiology, Vol. 432, No. 3, 07.08.1996, p. 538-545.

Research output: Contribution to journalArticle

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