Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks

Hiroyuki Kaiya, Ei Suke Saito, Tetsuya Tachibana, Mitsuhiro Furuse, Kenji Kangawa

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old layer chicks. After fasting, the plasma ghrelin levels increased from 21.3 ± 4.5 to 32.9 ± 5.0 fmol/ml, but once refed it returned to the control value. After fasting, the ghrelin mRNA and the peptide levels in the proventriculus increased, and ghrelin mRNA levels remained high but once refed the ghrelin content returned to the control level. Furthermore, in order to examine the effect of increased circulating ghrelin on food intake, a bolus intravenous injection of 500 pmol of chicken ghrelin was given to 8-day-old chicks. The ghrelin injection did not cause any significant changes in food intake. These results indicate that the levels of ghrelin and its mRNA with layer chicks are altered according to the feeding state and this in a similar manner as has been observed in mammals. Unlike in mammals, an increase in circulating ghrelin does not cause the promotion of food intake in chicks.

Original languageEnglish
Pages (from-to)247-259
Number of pages13
JournalDomestic Animal Endocrinology
Volume32
Issue number4
DOIs
Publication statusPublished - May 1 2007

Fingerprint

Proventriculus
ghrelin
proventriculus
Ghrelin
chicks
Messenger RNA
fasting
food intake
Fasting
Eating
Avian Stomach
Mammals
mammals
radioimmunoassays
intravenous injection
Intravenous Injections

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

Cite this

Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks. / Kaiya, Hiroyuki; Saito, Ei Suke; Tachibana, Tetsuya; Furuse, Mitsuhiro; Kangawa, Kenji.

In: Domestic Animal Endocrinology, Vol. 32, No. 4, 01.05.2007, p. 247-259.

Research output: Contribution to journalArticle

Kaiya, Hiroyuki ; Saito, Ei Suke ; Tachibana, Tetsuya ; Furuse, Mitsuhiro ; Kangawa, Kenji. / Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks. In: Domestic Animal Endocrinology. 2007 ; Vol. 32, No. 4. pp. 247-259.
@article{db9777ed0cc74fdf8ac62f6ab1bce99e,
title = "Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks",
abstract = "This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old layer chicks. After fasting, the plasma ghrelin levels increased from 21.3 ± 4.5 to 32.9 ± 5.0 fmol/ml, but once refed it returned to the control value. After fasting, the ghrelin mRNA and the peptide levels in the proventriculus increased, and ghrelin mRNA levels remained high but once refed the ghrelin content returned to the control level. Furthermore, in order to examine the effect of increased circulating ghrelin on food intake, a bolus intravenous injection of 500 pmol of chicken ghrelin was given to 8-day-old chicks. The ghrelin injection did not cause any significant changes in food intake. These results indicate that the levels of ghrelin and its mRNA with layer chicks are altered according to the feeding state and this in a similar manner as has been observed in mammals. Unlike in mammals, an increase in circulating ghrelin does not cause the promotion of food intake in chicks.",
author = "Hiroyuki Kaiya and Saito, {Ei Suke} and Tetsuya Tachibana and Mitsuhiro Furuse and Kenji Kangawa",
year = "2007",
month = "5",
day = "1",
doi = "10.1016/j.domaniend.2006.03.006",
language = "English",
volume = "32",
pages = "247--259",
journal = "Domestic Animal Endocrinology",
issn = "0739-7240",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Changes in ghrelin levels of plasma and proventriculus and ghrelin mRNA of proventriculus in fasted and refed layer chicks

AU - Kaiya, Hiroyuki

AU - Saito, Ei Suke

AU - Tachibana, Tetsuya

AU - Furuse, Mitsuhiro

AU - Kangawa, Kenji

PY - 2007/5/1

Y1 - 2007/5/1

N2 - This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old layer chicks. After fasting, the plasma ghrelin levels increased from 21.3 ± 4.5 to 32.9 ± 5.0 fmol/ml, but once refed it returned to the control value. After fasting, the ghrelin mRNA and the peptide levels in the proventriculus increased, and ghrelin mRNA levels remained high but once refed the ghrelin content returned to the control level. Furthermore, in order to examine the effect of increased circulating ghrelin on food intake, a bolus intravenous injection of 500 pmol of chicken ghrelin was given to 8-day-old chicks. The ghrelin injection did not cause any significant changes in food intake. These results indicate that the levels of ghrelin and its mRNA with layer chicks are altered according to the feeding state and this in a similar manner as has been observed in mammals. Unlike in mammals, an increase in circulating ghrelin does not cause the promotion of food intake in chicks.

AB - This is a test-report of ghrelin levels in plasma and proventriculus, the glandular portion of the avian stomach, by using a specific radioimmunoassay for acylated ghrelin, as well as the expression of the ghrelin gene in the proventriculus after a 12-h fasting period followed by a 6-h feeding period with 6-day-old layer chicks. After fasting, the plasma ghrelin levels increased from 21.3 ± 4.5 to 32.9 ± 5.0 fmol/ml, but once refed it returned to the control value. After fasting, the ghrelin mRNA and the peptide levels in the proventriculus increased, and ghrelin mRNA levels remained high but once refed the ghrelin content returned to the control level. Furthermore, in order to examine the effect of increased circulating ghrelin on food intake, a bolus intravenous injection of 500 pmol of chicken ghrelin was given to 8-day-old chicks. The ghrelin injection did not cause any significant changes in food intake. These results indicate that the levels of ghrelin and its mRNA with layer chicks are altered according to the feeding state and this in a similar manner as has been observed in mammals. Unlike in mammals, an increase in circulating ghrelin does not cause the promotion of food intake in chicks.

UR - http://www.scopus.com/inward/record.url?scp=33947251830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947251830&partnerID=8YFLogxK

U2 - 10.1016/j.domaniend.2006.03.006

DO - 10.1016/j.domaniend.2006.03.006

M3 - Article

VL - 32

SP - 247

EP - 259

JO - Domestic Animal Endocrinology

JF - Domestic Animal Endocrinology

SN - 0739-7240

IS - 4

ER -