Changes of tropomyosin isoforms during development of cross-fertilized sea urchin embryos

Takayuki Tobita, Tadashi Ishimoda-Takagi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Changes of tropomyosin isoforms during development of the sea urchin, Hemicentrotus pulcherrimus, were investigated using two-dimensional urea-shift gel electrophoresis. Tropomyosin isoforms included in the embryos were gradually increased after 2 cell stage and retained at a constant level after gastrula stage. To detect the tropomyosin isoforms derived from zygotic genomes, embryos cross-fertilized between H. pulcherrimus and Pseudocentrotus depressus gametes were prepared. Since tropomyosin isoforms from H. pulcherrimus eggs and from P. depressus eggs could be distinguished from each other on a two-dimensional eleotrophoretic gel, the paternal isoforms of tropomyosin in the cross-fertilized embryos, which were not included endogenously in the egg, could be regarded as products derived from zygotic genomes. The paternal isoforms of tropomyosin were detected first at around the gastrula stage in embryos cross-fertilized between H. pulcherrimus sperm and P. depressus eggs and also in the reverse combination of the gamete species. Muscle tropomyosins derived from H. pulcherrimus and P. depressus genomes were similarly detected in cross-fertilized embryos at the pluteic stage when the muscle tropomyosin appeared in sea urchin embryos.

Original languageEnglish
Pages (from-to)209-219
Number of pages11
JournalDevelopment Growth and Differentiation
Volume38
Issue number2
DOIs
Publication statusPublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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