Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

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6 Citations (Scopus)

Abstract

We demonstrate channel wall coating using a cellulose derivative on a poly-(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15°) and EOF was significantly suppressed (up to 4.0 × 10-6 cm2·V-1·s-1). Three proteins (20.5, 68.0, and 114.6 kDa) were successfidly separated on the 0.15% HPMC 90SH-coated channel walls with good reproducibility of migration time (RSD <1.75%) and high efficiency (theoretical plate number per meter: 2.62 × 105).

Original languageEnglish
Pages (from-to)4582-4589
Number of pages8
JournalELECTROPHORESIS
Volume28
Issue number24
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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