Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos

Yoshiko Takahashi, Tadayoshi Watanabe, Shinichi Nakagawa, Koichi Kawakami, Yuki Sato

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Citations (Scopus)

Abstract

The Tol2-mediated transposition approaches are novel techniques for the molecular manipulation of chicken embryos by which an exogenous gene can be integrated into the host genome. The integrated gene(s) can be stably expressed when driven by a ubiquitous promoter. This method can also be used for conditional expression when combined with the Tet-on system to achieve temporal control. The Tol2-transposition method allows for expression at stages even later than E5, which otherwise could not be analyzed with the conventional electroporation technique. These developmental stages are critical for organogenesis, where numerous tissues interact to generate the complex structures of functional organs. Thus, the techniques presented here are extremely useful for understanding how cells contribute to organogenesis at the molecular level.

Original languageEnglish
Title of host publicationAvian Embryology, 2nd Edition
EditorsMarianne Bronner-Fraser
Pages271-280
Number of pages10
DOIs
Publication statusPublished - May 14 2008
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume87
ISSN (Print)0091-679X

Fingerprint

Organogenesis
Tetracycline
Transgenes
Chickens
Embryonic Structures
Electroporation
Genes
Genome

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Takahashi, Y., Watanabe, T., Nakagawa, S., Kawakami, K., & Sato, Y. (2008). Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos. In M. Bronner-Fraser (Ed.), Avian Embryology, 2nd Edition (pp. 271-280). (Methods in Cell Biology; Vol. 87). https://doi.org/10.1016/S0091-679X(08)00214-8

Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos. / Takahashi, Yoshiko; Watanabe, Tadayoshi; Nakagawa, Shinichi; Kawakami, Koichi; Sato, Yuki.

Avian Embryology, 2nd Edition. ed. / Marianne Bronner-Fraser. 2008. p. 271-280 (Methods in Cell Biology; Vol. 87).

Research output: Chapter in Book/Report/Conference proceedingChapter

Takahashi, Y, Watanabe, T, Nakagawa, S, Kawakami, K & Sato, Y 2008, Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos. in M Bronner-Fraser (ed.), Avian Embryology, 2nd Edition. Methods in Cell Biology, vol. 87, pp. 271-280. https://doi.org/10.1016/S0091-679X(08)00214-8
Takahashi Y, Watanabe T, Nakagawa S, Kawakami K, Sato Y. Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos. In Bronner-Fraser M, editor, Avian Embryology, 2nd Edition. 2008. p. 271-280. (Methods in Cell Biology). https://doi.org/10.1016/S0091-679X(08)00214-8
Takahashi, Yoshiko ; Watanabe, Tadayoshi ; Nakagawa, Shinichi ; Kawakami, Koichi ; Sato, Yuki. / Chapter 14 Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos. Avian Embryology, 2nd Edition. editor / Marianne Bronner-Fraser. 2008. pp. 271-280 (Methods in Cell Biology).
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