Characteristic behavior of crowding macromolecules confined in cell-sized droplets

Miho Yanagisawa, Takahiro Sakaue, Kenichi Yoshikawa

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

We describe the unique behavior of long DNA molecules in a cell-sized confinement by using cell-sized water-in-oil droplets coated with a lipid layer. It is shown that DNA molecules exhibit specific localization on lipid membranes accompanied by conformational transitions depending on the spatial size of droplets as well as the chemical structure of lipids coating the droplets. Interestingly, such specific behavior of DNA molecules causes on/off switching of transcriptional activity. We demonstrate the effect of crowding by adapting various kinds of macromolecules with different electronic charges and flexibility. As recently reported, it is shown that gene expression is remarkably accelerated in cell-sized confinement. We theoretically discuss the abovementioned unique behavior of DNA molecules in a micro-confinement. Further study in comparison with the actual behavior of DNA molecules in a living cellular system is expected to be fruitful.

Original languageEnglish
Title of host publicationInternational Review of Cell and Molecular Biology
PublisherElsevier Inc.
Pages175-204
Number of pages30
DOIs
Publication statusPublished - Jan 1 2014

Publication series

NameInternational Review of Cell and Molecular Biology
Volume307
ISSN (Print)1937-6448

Fingerprint

Macromolecules
Molecules
DNA
Lipids
Membrane Lipids
Gene expression
Oils
Gene Expression
Coatings
Water

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yanagisawa, M., Sakaue, T., & Yoshikawa, K. (2014). Characteristic behavior of crowding macromolecules confined in cell-sized droplets. In International Review of Cell and Molecular Biology (pp. 175-204). (International Review of Cell and Molecular Biology; Vol. 307). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-800046-5.00007-2

Characteristic behavior of crowding macromolecules confined in cell-sized droplets. / Yanagisawa, Miho; Sakaue, Takahiro; Yoshikawa, Kenichi.

International Review of Cell and Molecular Biology. Elsevier Inc., 2014. p. 175-204 (International Review of Cell and Molecular Biology; Vol. 307).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yanagisawa, M, Sakaue, T & Yoshikawa, K 2014, Characteristic behavior of crowding macromolecules confined in cell-sized droplets. in International Review of Cell and Molecular Biology. International Review of Cell and Molecular Biology, vol. 307, Elsevier Inc., pp. 175-204. https://doi.org/10.1016/B978-0-12-800046-5.00007-2
Yanagisawa M, Sakaue T, Yoshikawa K. Characteristic behavior of crowding macromolecules confined in cell-sized droplets. In International Review of Cell and Molecular Biology. Elsevier Inc. 2014. p. 175-204. (International Review of Cell and Molecular Biology). https://doi.org/10.1016/B978-0-12-800046-5.00007-2
Yanagisawa, Miho ; Sakaue, Takahiro ; Yoshikawa, Kenichi. / Characteristic behavior of crowding macromolecules confined in cell-sized droplets. International Review of Cell and Molecular Biology. Elsevier Inc., 2014. pp. 175-204 (International Review of Cell and Molecular Biology).
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