Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver

Mitsuo Shimada, Yo Ichi Yamashita, Shinji Tanaka, Ken Shirabe, Kohji Nakazawa, Hiroyuki Ijima, Ryoichi Sakiyama, Junji Fukuda, Kazumori Funatsu, Keizo Sugimachi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background/Aims: Polyurethane foam (PUF)/ spheroid-culture can improve liver-specific functions of hepatoma cell line, Hep G2. Therefore, gene expression profile in the PUF/spheroid culture is hypothesized to be different from that in the monolayer culture. The aim of this study is to clarify the characteristic gene expression in PUF/spheroid-cultured Hep G2 cells, as a cell source for bioartificial liver (BAL), using microarray analysis. Methodology: Morphological change and liver specific functions of ammonia removal rate and albumin synthesis rate of Hep G2 were compared between in a monolayer or PUF/spheroid culture. Microarray analysis was performed using cDNA microarrays made in Hitachi Software Engineering Co., Ltd., (Yokohama, Japan), which contains a total of 1,281 cDNA clones. Results: The ammonia removal rate of Hep G2 spheroids increased to 369%, and the albumin synthesis rate of Hep G2 spheroids also increased 311% when compared with monolayer culture. In addition, the ammonia removal capacity of primary human hepatocytes in the PUF/spheroid culture was superior to that in the monolayer culture. The microarray analysis demonstrated that the PUF/spheroid-cultured Hep G2 cells expressed 39 up-regulated (more than 3.0-fold) and 31 down-regulated (less than 0.333-fold) genes. Among the 70 genes differentially expressed in PUF/spheroid cultured Hep G2 cells, subsets of glutathione S-transferase- and angio-tensin-related genes were drastically up-regulated, on the other hand, subsets of assigned for growth factor, glucocorticoid, and stress response, were down-regulated. Conclusions: Hepatoma cell line, Hep G2 cells in the PUF/spheroid culture is a promising hepatocyte source for BAL. Microarray analysis revealed a number of characteristic genes altered by the PUF/spheroid.

Original languageEnglish
Pages (from-to)814-820
Number of pages7
JournalHepato-gastroenterology
Volume54
Issue number75
Publication statusPublished - Apr 1 2007

Fingerprint

Artificial Liver
Hep G2 Cells
Hepatocellular Carcinoma
Gene Expression
Microarray Analysis
Ammonia
Cultured Cells
Genes
Hepatocytes
Albumins
polyurethane foam
Liver
Oligonucleotide Array Sequence Analysis
Glutathione Transferase
Transcriptome
Glucocorticoids
Intercellular Signaling Peptides and Proteins
Japan
Software
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver. / Shimada, Mitsuo; Yamashita, Yo Ichi; Tanaka, Shinji; Shirabe, Ken; Nakazawa, Kohji; Ijima, Hiroyuki; Sakiyama, Ryoichi; Fukuda, Junji; Funatsu, Kazumori; Sugimachi, Keizo.

In: Hepato-gastroenterology, Vol. 54, No. 75, 01.04.2007, p. 814-820.

Research output: Contribution to journalArticle

Shimada, M, Yamashita, YI, Tanaka, S, Shirabe, K, Nakazawa, K, Ijima, H, Sakiyama, R, Fukuda, J, Funatsu, K & Sugimachi, K 2007, 'Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver', Hepato-gastroenterology, vol. 54, no. 75, pp. 814-820.
Shimada, Mitsuo ; Yamashita, Yo Ichi ; Tanaka, Shinji ; Shirabe, Ken ; Nakazawa, Kohji ; Ijima, Hiroyuki ; Sakiyama, Ryoichi ; Fukuda, Junji ; Funatsu, Kazumori ; Sugimachi, Keizo. / Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver. In: Hepato-gastroenterology. 2007 ; Vol. 54, No. 75. pp. 814-820.
@article{e9bd1e0dee514720ae3b17f7fdedb0d4,
title = "Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver",
abstract = "Background/Aims: Polyurethane foam (PUF)/ spheroid-culture can improve liver-specific functions of hepatoma cell line, Hep G2. Therefore, gene expression profile in the PUF/spheroid culture is hypothesized to be different from that in the monolayer culture. The aim of this study is to clarify the characteristic gene expression in PUF/spheroid-cultured Hep G2 cells, as a cell source for bioartificial liver (BAL), using microarray analysis. Methodology: Morphological change and liver specific functions of ammonia removal rate and albumin synthesis rate of Hep G2 were compared between in a monolayer or PUF/spheroid culture. Microarray analysis was performed using cDNA microarrays made in Hitachi Software Engineering Co., Ltd., (Yokohama, Japan), which contains a total of 1,281 cDNA clones. Results: The ammonia removal rate of Hep G2 spheroids increased to 369{\%}, and the albumin synthesis rate of Hep G2 spheroids also increased 311{\%} when compared with monolayer culture. In addition, the ammonia removal capacity of primary human hepatocytes in the PUF/spheroid culture was superior to that in the monolayer culture. The microarray analysis demonstrated that the PUF/spheroid-cultured Hep G2 cells expressed 39 up-regulated (more than 3.0-fold) and 31 down-regulated (less than 0.333-fold) genes. Among the 70 genes differentially expressed in PUF/spheroid cultured Hep G2 cells, subsets of glutathione S-transferase- and angio-tensin-related genes were drastically up-regulated, on the other hand, subsets of assigned for growth factor, glucocorticoid, and stress response, were down-regulated. Conclusions: Hepatoma cell line, Hep G2 cells in the PUF/spheroid culture is a promising hepatocyte source for BAL. Microarray analysis revealed a number of characteristic genes altered by the PUF/spheroid.",
author = "Mitsuo Shimada and Yamashita, {Yo Ichi} and Shinji Tanaka and Ken Shirabe and Kohji Nakazawa and Hiroyuki Ijima and Ryoichi Sakiyama and Junji Fukuda and Kazumori Funatsu and Keizo Sugimachi",
year = "2007",
month = "4",
day = "1",
language = "English",
volume = "54",
pages = "814--820",
journal = "Acta hepato-splenologica",
issn = "0172-6390",
publisher = "H.G.E. Update Medical Publishing Ltd.",
number = "75",

}

TY - JOUR

T1 - Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver

AU - Shimada, Mitsuo

AU - Yamashita, Yo Ichi

AU - Tanaka, Shinji

AU - Shirabe, Ken

AU - Nakazawa, Kohji

AU - Ijima, Hiroyuki

AU - Sakiyama, Ryoichi

AU - Fukuda, Junji

AU - Funatsu, Kazumori

AU - Sugimachi, Keizo

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Background/Aims: Polyurethane foam (PUF)/ spheroid-culture can improve liver-specific functions of hepatoma cell line, Hep G2. Therefore, gene expression profile in the PUF/spheroid culture is hypothesized to be different from that in the monolayer culture. The aim of this study is to clarify the characteristic gene expression in PUF/spheroid-cultured Hep G2 cells, as a cell source for bioartificial liver (BAL), using microarray analysis. Methodology: Morphological change and liver specific functions of ammonia removal rate and albumin synthesis rate of Hep G2 were compared between in a monolayer or PUF/spheroid culture. Microarray analysis was performed using cDNA microarrays made in Hitachi Software Engineering Co., Ltd., (Yokohama, Japan), which contains a total of 1,281 cDNA clones. Results: The ammonia removal rate of Hep G2 spheroids increased to 369%, and the albumin synthesis rate of Hep G2 spheroids also increased 311% when compared with monolayer culture. In addition, the ammonia removal capacity of primary human hepatocytes in the PUF/spheroid culture was superior to that in the monolayer culture. The microarray analysis demonstrated that the PUF/spheroid-cultured Hep G2 cells expressed 39 up-regulated (more than 3.0-fold) and 31 down-regulated (less than 0.333-fold) genes. Among the 70 genes differentially expressed in PUF/spheroid cultured Hep G2 cells, subsets of glutathione S-transferase- and angio-tensin-related genes were drastically up-regulated, on the other hand, subsets of assigned for growth factor, glucocorticoid, and stress response, were down-regulated. Conclusions: Hepatoma cell line, Hep G2 cells in the PUF/spheroid culture is a promising hepatocyte source for BAL. Microarray analysis revealed a number of characteristic genes altered by the PUF/spheroid.

AB - Background/Aims: Polyurethane foam (PUF)/ spheroid-culture can improve liver-specific functions of hepatoma cell line, Hep G2. Therefore, gene expression profile in the PUF/spheroid culture is hypothesized to be different from that in the monolayer culture. The aim of this study is to clarify the characteristic gene expression in PUF/spheroid-cultured Hep G2 cells, as a cell source for bioartificial liver (BAL), using microarray analysis. Methodology: Morphological change and liver specific functions of ammonia removal rate and albumin synthesis rate of Hep G2 were compared between in a monolayer or PUF/spheroid culture. Microarray analysis was performed using cDNA microarrays made in Hitachi Software Engineering Co., Ltd., (Yokohama, Japan), which contains a total of 1,281 cDNA clones. Results: The ammonia removal rate of Hep G2 spheroids increased to 369%, and the albumin synthesis rate of Hep G2 spheroids also increased 311% when compared with monolayer culture. In addition, the ammonia removal capacity of primary human hepatocytes in the PUF/spheroid culture was superior to that in the monolayer culture. The microarray analysis demonstrated that the PUF/spheroid-cultured Hep G2 cells expressed 39 up-regulated (more than 3.0-fold) and 31 down-regulated (less than 0.333-fold) genes. Among the 70 genes differentially expressed in PUF/spheroid cultured Hep G2 cells, subsets of glutathione S-transferase- and angio-tensin-related genes were drastically up-regulated, on the other hand, subsets of assigned for growth factor, glucocorticoid, and stress response, were down-regulated. Conclusions: Hepatoma cell line, Hep G2 cells in the PUF/spheroid culture is a promising hepatocyte source for BAL. Microarray analysis revealed a number of characteristic genes altered by the PUF/spheroid.

UR - http://www.scopus.com/inward/record.url?scp=34250198579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250198579&partnerID=8YFLogxK

M3 - Article

C2 - 17591070

AN - SCOPUS:34250198579

VL - 54

SP - 814

EP - 820

JO - Acta hepato-splenologica

JF - Acta hepato-splenologica

SN - 0172-6390

IS - 75

ER -