Characteristics of apparent kinetic parameters in a packed-bed immobilized enzyme reactor

Fumihide Shiraishi, T. Hasegawa, S. Kasai, N. Makishita, H. Miyakawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The design equation for a packed-bed immobilized enzyme reactor is expressed in terms of apparent kinetic parameters and the relationship between the exit concentration of substrate consumed and the logarithm of the exit unconverted fraction of substrate is studied. Agreement of calculated values with experimental data indicate that this relationship is not linear but is convex downward. Furthermore, simulation is made to characterize the apparent kinetic parameters. The results show that with increasing flow rates of a reaction mixture, the apparent kinetic parameters decrease rapidly towards their respective intrinsic values. As the entrance substrate concentration increases, on the other hand, these values initially increase, pass through maximums, and finally decrease towards their intrinsic values. These variations are found to occur remarkably with the apparent Michaelis constant.

Original languageEnglish
Pages (from-to)2847-2852
Number of pages6
JournalChemical Engineering Science
Volume51
Issue number11
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Immobilized Enzymes
Packed beds
Kinetic parameters
Enzymes
Substrates
Flow rate

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Characteristics of apparent kinetic parameters in a packed-bed immobilized enzyme reactor. / Shiraishi, Fumihide; Hasegawa, T.; Kasai, S.; Makishita, N.; Miyakawa, H.

In: Chemical Engineering Science, Vol. 51, No. 11, 01.01.1996, p. 2847-2852.

Research output: Contribution to journalArticle

Shiraishi, Fumihide ; Hasegawa, T. ; Kasai, S. ; Makishita, N. ; Miyakawa, H. / Characteristics of apparent kinetic parameters in a packed-bed immobilized enzyme reactor. In: Chemical Engineering Science. 1996 ; Vol. 51, No. 11. pp. 2847-2852.
@article{497eca4a5728442d8781e6555920f370,
title = "Characteristics of apparent kinetic parameters in a packed-bed immobilized enzyme reactor",
abstract = "The design equation for a packed-bed immobilized enzyme reactor is expressed in terms of apparent kinetic parameters and the relationship between the exit concentration of substrate consumed and the logarithm of the exit unconverted fraction of substrate is studied. Agreement of calculated values with experimental data indicate that this relationship is not linear but is convex downward. Furthermore, simulation is made to characterize the apparent kinetic parameters. The results show that with increasing flow rates of a reaction mixture, the apparent kinetic parameters decrease rapidly towards their respective intrinsic values. As the entrance substrate concentration increases, on the other hand, these values initially increase, pass through maximums, and finally decrease towards their intrinsic values. These variations are found to occur remarkably with the apparent Michaelis constant.",
author = "Fumihide Shiraishi and T. Hasegawa and S. Kasai and N. Makishita and H. Miyakawa",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/0009-2509(96)00163-7",
language = "English",
volume = "51",
pages = "2847--2852",
journal = "Chemical Engineering Science",
issn = "0009-2509",
publisher = "Elsevier BV",
number = "11",

}

TY - JOUR

T1 - Characteristics of apparent kinetic parameters in a packed-bed immobilized enzyme reactor

AU - Shiraishi, Fumihide

AU - Hasegawa, T.

AU - Kasai, S.

AU - Makishita, N.

AU - Miyakawa, H.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The design equation for a packed-bed immobilized enzyme reactor is expressed in terms of apparent kinetic parameters and the relationship between the exit concentration of substrate consumed and the logarithm of the exit unconverted fraction of substrate is studied. Agreement of calculated values with experimental data indicate that this relationship is not linear but is convex downward. Furthermore, simulation is made to characterize the apparent kinetic parameters. The results show that with increasing flow rates of a reaction mixture, the apparent kinetic parameters decrease rapidly towards their respective intrinsic values. As the entrance substrate concentration increases, on the other hand, these values initially increase, pass through maximums, and finally decrease towards their intrinsic values. These variations are found to occur remarkably with the apparent Michaelis constant.

AB - The design equation for a packed-bed immobilized enzyme reactor is expressed in terms of apparent kinetic parameters and the relationship between the exit concentration of substrate consumed and the logarithm of the exit unconverted fraction of substrate is studied. Agreement of calculated values with experimental data indicate that this relationship is not linear but is convex downward. Furthermore, simulation is made to characterize the apparent kinetic parameters. The results show that with increasing flow rates of a reaction mixture, the apparent kinetic parameters decrease rapidly towards their respective intrinsic values. As the entrance substrate concentration increases, on the other hand, these values initially increase, pass through maximums, and finally decrease towards their intrinsic values. These variations are found to occur remarkably with the apparent Michaelis constant.

UR - http://www.scopus.com/inward/record.url?scp=0030168501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030168501&partnerID=8YFLogxK

U2 - 10.1016/0009-2509(96)00163-7

DO - 10.1016/0009-2509(96)00163-7

M3 - Article

AN - SCOPUS:0030168501

VL - 51

SP - 2847

EP - 2852

JO - Chemical Engineering Science

JF - Chemical Engineering Science

SN - 0009-2509

IS - 11

ER -