Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)–mass spectrometric (MS) detection of synthetic di-to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di-to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 – 300 Å2, regardless of hydrophobicity and derivatization methods.
|Number of pages||4|
|Publication status||Published - 2021|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry