Characteristics of Electrospray-Ionization Detection of Synthetic Di-to Penta-Oligopeptides by Amine Derivatizations

Weilin Shen; Kiyomi Ono; Mitsuru Tanaka; Toshiro Matsui

doi:10.2116/analsci.21N016

Characteristics of Electrospray-Ionization Detection of Synthetic Di-to Penta-Oligopeptides by Amine Derivatizations

Weilin Shen, Kiyomi Ono, Mitsuru Tanaka, Toshiro Matsui

Research output: Contribution to journal › Article › peer-review

Abstract

Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)–mass spectrometric (MS) detection of synthetic di-to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di-to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 – 300 Å2, regardless of hydrophobicity and derivatization methods.

Original language	English
Pages (from-to)	1629-1632
Number of pages	4
Journal	analytical sciences
Volume	37
Issue number	11
DOIs	https://doi.org/10.2116/analsci.21N016
Publication status	Published - 2021

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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title = "Characteristics of Electrospray-Ionization Detection of Synthetic Di-to Penta-Oligopeptides by Amine Derivatizations",

abstract = "Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)–mass spectrometric (MS) detection of synthetic di-to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di-to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 – 300 {\AA}2, regardless of hydrophobicity and derivatization methods.",

author = "Weilin Shen and Kiyomi Ono and Mitsuru Tanaka and Toshiro Matsui",

note = "Funding Information: This study was supported in part by a JSPS Research Fellowship for Young Scientists awarded to W. S. (No. 19J20948). Publisher Copyright: {\textcopyright} The Japan Society for Analytical Chemistry",

year = "2021",

doi = "10.2116/analsci.21N016",

language = "English",

volume = "37",

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T1 - Characteristics of Electrospray-Ionization Detection of Synthetic Di-to Penta-Oligopeptides by Amine Derivatizations

AU - Shen, Weilin

AU - Ono, Kiyomi

AU - Tanaka, Mitsuru

AU - Matsui, Toshiro

N1 - Funding Information: This study was supported in part by a JSPS Research Fellowship for Young Scientists awarded to W. S. (No. 19J20948). Publisher Copyright: © The Japan Society for Analytical Chemistry

PY - 2021

Y1 - 2021

N2 - Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)–mass spectrometric (MS) detection of synthetic di-to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di-to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 – 300 Å2, regardless of hydrophobicity and derivatization methods.

AB - Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)–mass spectrometric (MS) detection of synthetic di-to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di-to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 – 300 Å2, regardless of hydrophobicity and derivatization methods.

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Characteristics of Electrospray-Ionization Detection of Synthetic Di-to Penta-Oligopeptides by Amine Derivatizations

Abstract

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