Characteristics of microbial communities in crustal fluids in a deep-sea hydrothermal field of the Suiyo Seamount

Shingo Kato, Michiyuki Nakawake, Junko Kita, Toshiro Yamanaka, Motoo Utsumi, Kei Okamura, Jun Ichiro Ishibashi, Moriya Ohkuma, Akihiko Yamagishi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To directly access the sub-seafloor microbial communities, seafloor drilling has been done in a deep-sea hydrothermal field of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific. In the present study, crustal fluids were collected from the boreholes, and the bacterial and archaeal communities in the fluids were investigated by culture-independent molecular analysis based on 16S rRNA gene sequences. Bottom seawater, sands, rocks, sulfide mound, and chimneys were also collected around the boreholes and analyzed for comparisons. Comprehensive analysis revealed the characteristics of the microbial community composition in the crustal fluids. Phylotypes closely related to cultured species, e.g., Alteromonas, Halomonas, Marinobacter, were relatively abundant in some crustal fluid samples, whereas the phylotypes related to Pelagibacter and the SUP05-group were relatively abundant in the seawater samples. Phylotypes related to other uncultured environmental clones in Alphaproteobacteria and Gammaproteobacteria were relatively abundant in the sand, rock, sulfide mound, and chimney samples. Furthermore, comparative analysis with previous studies of the Suiyo Seamount crustal fluids indicates the change in the microbial community composition for 3 years. Our results provide novel insights into the characteristics of the microbial communities in crustal fluids beneath a deep-sea hydrothermal field.

Original languageEnglish
JournalFrontiers in Microbiology
Volume4
Issue numberAPR
DOIs
Publication statusPublished - Jan 1 2013

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Seawater
Sulfides
Oceans and Seas
Marinobacter
Alteromonas
Halomonas
Alphaproteobacteria
Gammaproteobacteria
rRNA Genes
Clone Cells

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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Characteristics of microbial communities in crustal fluids in a deep-sea hydrothermal field of the Suiyo Seamount. / Kato, Shingo; Nakawake, Michiyuki; Kita, Junko; Yamanaka, Toshiro; Utsumi, Motoo; Okamura, Kei; Ishibashi, Jun Ichiro; Ohkuma, Moriya; Yamagishi, Akihiko.

In: Frontiers in Microbiology, Vol. 4, No. APR, 01.01.2013.

Research output: Contribution to journalArticle

Kato, Shingo ; Nakawake, Michiyuki ; Kita, Junko ; Yamanaka, Toshiro ; Utsumi, Motoo ; Okamura, Kei ; Ishibashi, Jun Ichiro ; Ohkuma, Moriya ; Yamagishi, Akihiko. / Characteristics of microbial communities in crustal fluids in a deep-sea hydrothermal field of the Suiyo Seamount. In: Frontiers in Microbiology. 2013 ; Vol. 4, No. APR.
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