Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan

Munenori Furuta; Takayuki Nasu; Kouichi Umeki; Duc Hoang Minh; Ken Ichi Honjoh; Takahisa Miyamoto

doi:10.4265/bio.22.213

Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan

Munenori Furuta, Takayuki Nasu, Kouichi Umeki, Duc Hoang Minh, Ken Ichi Honjoh, Takahisa Miyamoto

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The aim was to isolate Campylobacter jejuni- specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 (86.7%) retail chicken meat samples (skin and liver) by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: Active against 67.4% of the 46 C. jejuni strains tested. The other phage isolates showed different lytic spectra. Because phages PHC5, PHC10, PHC19, PHC22, and PHC25 possess an icosahedral head and a contracted tail, they seem to be members of the Myoviridae family. Effects of 19 phage isolates on viability of C. jejuni were investigated. These phages reduced viable counts of C. jejuni by 1-3 log after 6-12 h of incubation at 42C as compared to the initial counts. The C. jejuni L26 was found to be suitable as a host because of the wide hosting range. The phages isolated in this study seem to be promising biocontrol agents against C. jejuni in food.

Original language	English
Pages (from-to)	213-221
Number of pages	9
Journal	Biocontrol Science
Volume	22
Issue number	4
DOIs	https://doi.org/10.4265/bio.22.213
Publication status	Published - 2017

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

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10.4265/bio.22.213

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title = "Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan",

abstract = "The aim was to isolate Campylobacter jejuni- specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 (86.7%) retail chicken meat samples (skin and liver) by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: Active against 67.4% of the 46 C. jejuni strains tested. The other phage isolates showed different lytic spectra. Because phages PHC5, PHC10, PHC19, PHC22, and PHC25 possess an icosahedral head and a contracted tail, they seem to be members of the Myoviridae family. Effects of 19 phage isolates on viability of C. jejuni were investigated. These phages reduced viable counts of C. jejuni by 1-3 log after 6-12 h of incubation at 42C as compared to the initial counts. The C. jejuni L26 was found to be suitable as a host because of the wide hosting range. The phages isolated in this study seem to be promising biocontrol agents against C. jejuni in food.",

author = "Munenori Furuta and Takayuki Nasu and Kouichi Umeki and Minh, {Duc Hoang} and Honjoh, {Ken Ichi} and Takahisa Miyamoto",

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AU - Furuta, Munenori

AU - Nasu, Takayuki

AU - Umeki, Kouichi

AU - Minh, Duc Hoang

AU - Honjoh, Ken Ichi

AU - Miyamoto, Takahisa

N1 - Funding Information: 　This work was supported by JSPS KAKENHI grant number JP 26350114.

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AB - The aim was to isolate Campylobacter jejuni- specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 (86.7%) retail chicken meat samples (skin and liver) by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: Active against 67.4% of the 46 C. jejuni strains tested. The other phage isolates showed different lytic spectra. Because phages PHC5, PHC10, PHC19, PHC22, and PHC25 possess an icosahedral head and a contracted tail, they seem to be members of the Myoviridae family. Effects of 19 phage isolates on viability of C. jejuni were investigated. These phages reduced viable counts of C. jejuni by 1-3 log after 6-12 h of incubation at 42C as compared to the initial counts. The C. jejuni L26 was found to be suitable as a host because of the wide hosting range. The phages isolated in this study seem to be promising biocontrol agents against C. jejuni in food.

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Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan

Abstract

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